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Towards Novel Graphene-Enabled Diagnostic Assays with Improved Signal-to-Noise Ratio

Published online by Cambridge University Press:  13 June 2017

Savannah J. Afsahi ,
Lauren E. Locascio ,
Deng Pan ,
Yingning Gao ,
Amy E. Walker ,
Francie E. Barron ,
Brett R. Goldsmith  and
Mitchell B. Lerner
Savannah J. Afsahi
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
Lauren E. Locascio
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
Deng Pan
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
Yingning Gao
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
Amy E. Walker
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
Francie E. Barron
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
Brett R. Goldsmith
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
Mitchell B. Lerner*
Affiliation:
Nanomedical Diagnostics Inc., 6185 Cornerstone Court East Suite #110, San Diego, CA92121, U.S.A.
*
*(Email: mlerner@nanomedicaldiagnostics.com)
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Abstract

Large numbers of high quality graphene transistors were fabricated by chemical vapor deposition and packaged into a standard electronics assembly, enabling the readout of graphene properties on the benchtop. After chemical functionalization, these sensors demonstrate sensitivity into the pM range to inflammation (IL6) and Zika virus (ZIKV NS1) biomarkers. Signal-to-noise ratio (SNR) of graphene biosensors is over an order of magnitude greater than established diagnostic and biophysical assays, namely ELISA and BLI respectively. High precision measurements of protein kinetics captured using this technology, commercially available as the AGILE R100, are comparable to both clinical diagnostic and state-of-the-art biomolecule characterization tools. These results demonstrate that graphene-based platforms are highly attractive biological sensors for next generation diagnostics.

Keywords

sensorbiomedicalelectronic material
Type
Articles
Information
MRS Advances , Volume 2 , Issue 60: Nanomaterials , 2017 , pp. 3733 - 3739
Copyright
Copyright © Materials Research Society 2017 

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