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Biofunctionalized nanodot zirconia-based efficient biosensing platform for noninvasive oral cancer detection

Published online by Cambridge University Press:  30 September 2020

Suveen Kumar ,
Dipti Chauhan ,
Venkatesan Renugopalakrishnan  and
Bansi D. Malhotra
Suveen Kumar
Affiliation:
Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi110042, India Department of Chemistry, University of Delhi, Delhi110007, India
Dipti Chauhan
Affiliation:
Department of Chemistry, University of Delhi, Delhi110007, India
Venkatesan Renugopalakrishnan
Affiliation:
Boston Children's Hospital, Harvard Medical School, Boston, MA02115, USA Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA02115, USA
Bansi D. Malhotra*
Affiliation:
Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi110042, India
*
Address all correspondence to Bansi D. Malhotra at bansi.malhotra@gmail.com
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Abstract

The authors report results of the studies relating to the synthesis of nanodot zirconia that has been utilized for the fabrication of electrochemical biosensing platform for the detection of CYFRA-21-1 biomarker, secreted in saliva samples of oral cancer patients. For the synthesis of nanodot zirconia (ndZrO2), the hydrothermal process was used and further functionalized with 3-aminopropyl triethoxysilane (APTES). Electrophoretic deposition technique was employed for its deposition onto the ITO electrode. The EDC-NHS reaction was used for anti-CYFRA-21-1 immobilization and bovine serum albumin (BSA) was used for blocking of the nonspecific binding sites. The fabricated biosensing platform (BSA/anti-CYFRA-21-1/APTES/ndZrO2/ITO) exhibited a wide linear detection range (0.5–50 ng/mL) with excellent sensitivity (0.53 μA mL/ng cm2).

Type
Research Letters
Information
MRS Communications , Volume 10 , Issue 4 , December 2020 , pp. 652 - 659
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Copyright
Copyright © The Author(s), 2020, published on behalf of Materials Research Society by Cambridge University Press

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