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Development of a Porous Silicon Based Biosensor

Published online by Cambridge University Press:  09 August 2011

Keiki-Pua S. Dancil ,
Douglas P. Greiner  and
Michael J. Sailor
Keiki-Pua S. Dancil
Affiliation:
Department of Chemistry and Biochemistry, The University of California San Diego, La Jolla, CA 92093-0358
Douglas P. Greiner
Affiliation:
Department of Chemistry and Biochemistry, The University of California San Diego, La Jolla, CA 92093-0358
Michael J. Sailor
Affiliation:
Department of Chemistry and Biochemistry, The University of California San Diego, La Jolla, CA 92093-0358
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Abstract

In this paper we demonstrate that porous silicon (PS) can be used as an immobilization matrix and a transducer for biosensor applications. Thin layers of PS were fabricated showing fine structure in their reflection spectra, characteristic of longitudinal optical cavity modes, or Fabry-Perot interference fringes. The PS surface was modified by covalently bonding streptavidin to a heterobifunctional linker immobilized to the surface using common silane chemistry. The mode spacing and wavelength in the interference spectrum was modified, by displacing buffer and introducing proteins into the PS layer. Protein-protein interactions between immobilized Streptavidin and biotinylated Protein A followed by Protein A and IgG were detected. The surface was regenerated during the course of the experiment showing reversibility of the sensor at the third layer.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 536: Symposium F – Microcrystalline & Nanocrystalline Semiconductors - 1998 , 1998 , 557
Copyright
Copyright © Materials Research Society 1999

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References

(1) Lin, V. S.-Y.; Motesharei, K.; Dancil, K.-P. S.; Sailor, M. J.; Ghadiri, M. R. Science 1997, 278, 840–843.Google Scholar
(2) Rossi, B. Optics; Addison-Wesley: Reading, MA, 1957.Google Scholar
(3) Hermanson, G. T. Bioconjugate Techniques; Academic Press, Inc.: San Diego, 1996.Google Scholar
(4) Penefsky, H. S. Methods Enzymol. 1979, 56, 527–530.Google Scholar
(5) Janshoff, A.; Dancil, K.-P. S.; Steinem, C.; Greiner, D. P.; Lin, V. S.-Y.; Gurtner, C.; Motesharei, K.; Sailor, M. J.; Ghadiri, M. R. Journal of the American Chemical Society 1998, 120, 12108–12116.Google Scholar