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Investigation of Nanofibrillar Influence on Cell-Cell Interactions of Astrocytes by Epi-fluorescence and Atomic Force Microscopies

Published online by Cambridge University Press:  02 March 2011

Volkan M. Tiryaki ,
Virginia M. Ayres ,
Adeel A. Khan ,
Dexter A. Flowers ,
Ijaz Ahmed ,
Roberto Delgado-Rivera  and
Sally Meiners
Volkan M. Tiryaki
Affiliation:
College of Engineering, Michigan State University, East Lansing MI 48824
Virginia M. Ayres
Affiliation:
College of Engineering, Michigan State University, East Lansing MI 48824
Adeel A. Khan
Affiliation:
College of Engineering, Western Michigan University, Kalamazoo, MI 49008
Dexter A. Flowers
Affiliation:
College of Engineering, Wayne State University, Detroit, MI 48202
Ijaz Ahmed
Affiliation:
College of Engineering, Rutgers, State University of New Jersey, Piscataway, NJ 08854
Roberto Delgado-Rivera
Affiliation:
Department of Chemistry and Chemical Biology, Rutgers, State University of New Jersey, Piscataway, NJ 08854
Sally Meiners
Affiliation:
117 Bedford Court, Piscataway, NJ, 08854
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Abstract

Long distance intercellular communication between astrocytes on nanofibrillar and planar surfaces was investigated by epi-fluorescence microscopy and atomic force microscopy. We found that astrocytes on nanofibrillar surfaces and astrocytes on planar surfaces diverged in apparent cell-cell contact structures. Astrocytes on nanofibrillar surfaces exhibited a “single cellular process” response, while astrocytes on planar surfaces exhibited a filopodial network response. The possibility that astrocytes can sense their geometrical environment and form different cell-to-cell contacts on nanofibrillar versus planar surfaces, with activation of different signaling pathways, is discussed.

Keywords

cellular (material form)scanning probe microscopy (SPM)nanoscale
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1316: Symposium QQ – Nanofunctional Materials, Nanostructures and Nanodevices for Biomedical Applications II , 2011 , mrsf10-1316-qq09-16
Copyright
Copyright © Materials Research Society 2011

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References

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