Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-10T13:24:31.914Z Has data issue: false hasContentIssue false
  • English
  • Français

Iron oxide crystal formation on a substrate modified with the Mms6 protein from magnetotactic bacteria

Published online by Cambridge University Press:  31 January 2011

Atsushi Arakaki ,
Fukashi Masuda  and
Tadashi Matsunaga
Atsushi Arakaki
Affiliation:
arakakia@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, Tokyo, Japan
Fukashi Masuda
Affiliation:
50006641226@st.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, Tokyo, Japan
Tadashi Matsunaga
Affiliation:
tmatsuna@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, Tokyo, Japan
Get access

Abstract

Mms6 is a small acidic protein which is tightly bound to magnetite in the bacterium Magnetospirillum magneticum AMB-1. Mms6 has been previously shown to promote iron-binding capacity as well as modulate the size and morphology of magnetic iron oxide crystals in vitro. In this study, we synthesized iron oxide crystals by using a monolayer-modified substrate. A self-assembled monolayer of octadecyltrimethoxysilane was modified on a silicon substrate. Recombinant Mms6 protein was attached to the substrate through the hydrophobic interactions between the protein molecules and the monolayer. The immobilization of protein molecules on the substrate surface was confirmed by fluorescent labeling of these molecules and subsequent fluorescence microscopy. This protein-modified substrate was then used as a template for iron oxide crystal formation in a ferrous solution. Scanning electron microscopy revealed site-specific formation of iron oxide crystals in substrate regions with immobilized proteins. This use of proteins might provide an alternative method for the bottom-up fabrication of nano-sized magnetic particles.

Keywords

biomaterialbiologicalmagnetic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1187: Symposium KK – Structure-Property Relationships in Biomineralized and Biomimetic Composites , 2009 , 1187-KK03-08
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Eerenstein, W., Kalev, L., Niesen, L. et al., Journal of Magnetism and Magnetic Materials 258, 73 (2003).10.1016/S0304-8853(02)01109-5Google Scholar
2 Ohta, S. and Terada, A., Thin Solid Films 143 (1), 73 (1986).10.1016/0040-6090(86)90148-3Google Scholar
3 Masterson, H. J., Lunney, J. G., Coey, J. M. D. et al., Journal of Magnetism and Magnetic Materials 115 (2-3), 155 (1992).10.1016/0304-8853(92)90046-QGoogle Scholar
4 Chiba, M., Morio, K., and Koizumi, Y., Journal of Magnetism and Magnetic Materials 239 (1-3), 457 (2002).10.1016/S0304-8853(01)00645-XGoogle Scholar
5 Blakemore, R., Science 190 (4212), 377 (1975).10.1126/science.170679Google Scholar
6 Arakaki, A., Nakazawa, H., Nemoto, M. et al., Journal of the Royal Society Interface 5 (26), 977 (2008); D. A. Bazylinski and R. B. Frankel, Nat. Rev. Microbiol. 2 (3), 217 (2004).10.1098/rsif.2008.0170Google Scholar
7 Arakaki, A., Webb, J., and Matsunaga, T., J. Biol. Chem. 278 (10), 8745 (2003).10.1074/jbc.M211729200Google Scholar
8 Amemiya, Y., Arakaki, A., Staniland, S. S. et al., Biomaterials 28 (35), 5381 (2007).10.1016/j.biomaterials.2007.07.051Google Scholar
9 Aizenberg, J., Advanced Materials 16 (15), 1295 (2004).10.1002/adma.200400759Google Scholar
10 Kisailus, D., Truong, Q., Amemiya, Y. et al., Proc Natl Acad Sci U S A 103 (15), 5652 (2006).10.1073/pnas.0508488103Google Scholar
11 Arakaki, A., Hideshima, S., Nakagawa, T. et al., Biotechnology and Bioengineering 88 (4), 543 (2004).10.1002/bit.20262Google Scholar
12 Oh, C. Y., Oh, J. H., and Ko, T., IEEE Transactions on Magnetics 38 (5), 3018 (2002).Google Scholar
13 Niwa, D., Omichi, K., Motohashi, N. et al., Chemistry Letters 33 (2), 176 (2004).10.1246/cl.2004.176Google Scholar
14 Mooney, J. F., Hunt, A. J., McIntosh, J. R. et al., Proceedings of the National Academy of Sciences of the United States of America 93 (22), 12287 (1996).10.1073/pnas.93.22.12287Google Scholar
15 Fair, B. D. and Jamieson, A. M., Journal of Colloid and Interface Science 77 (2), 525 (1980).10.1016/0021-9797(80)90325-2Google Scholar