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A Novel Self-Assembled Collagenous Matrix which Serves as a Template for Oriented Growth of Hydroxyapatite Crystal

Published online by Cambridge University Press:  15 February 2011

D. L. Christiansen ,
G. D. Pins ,
E. K. Huang  and
F. H. Silver
D. L. Christiansen
Affiliation:
University of Medicine and Dentistry of New Jersey – Robert Wood Johnson Medical School, Dept. Pathology – Division of Biomaterials, 675 Hoes Lane, Piscataway, NJ 08854, christia@rwja.umdnj.edu
G. D. Pins
Affiliation:
University of Medicine and Dentistry of New Jersey – Robert Wood Johnson Medical School, Dept. Pathology – Division of Biomaterials, 675 Hoes Lane, Piscataway, NJ 08854, christia@rwja.umdnj.edu
E. K. Huang
Affiliation:
University of Medicine and Dentistry of New Jersey – Robert Wood Johnson Medical School, Dept. Pathology – Division of Biomaterials, 675 Hoes Lane, Piscataway, NJ 08854, christia@rwja.umdnj.edu
F. H. Silver
Affiliation:
University of Medicine and Dentistry of New Jersey – Robert Wood Johnson Medical School, Dept. Pathology – Division of Biomaterials, 675 Hoes Lane, Piscataway, NJ 08854, christia@rwja.umdnj.edu
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Abstract

Collagen fibers self-assembled from solutions of molecular type I collagen were mineralized at pH 9.5, by exposure to super-saturated solutions of calcium and phosphate for a one week period in a double diffusion chamber. Uniaxial tensile mechanical properties increased with mineralization and electron microscopy of the mineral formed within the fiber was morphologically similar to the mineral phase of calcified tissues. Selected area electron diffraction confirms the presence of hydroxyapatite crystal. Further, the aligned fibrillar substructure serves as a template for the orientation of the c-axis diffraction maxima of the hydroxyapatite. These results indicate that an aligned system composed exclusively of selfassembled type I collagen fibrils serves as a scaffold for oriented growth of mineral analogous to calcification in vertebrate bone.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 414: Symposium Z – Thin Films and Surfaces for Bioactivity and Biomedical Applications , 1995 , 29
Copyright
Copyright © Materials Research Society 1996

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