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Silicon Quantum Dots Functionalized for siRNA Delivery

Published online by Cambridge University Press:  01 February 2011

Klein Stefanie ,
Oliver Zolk ,
Falk Schrödl  and
Carola Kryschi
Klein Stefanie
Affiliation:
stefanie.klein@chemie.uni-erlangen.de, University of Erlangen, Physical Chemistry I, Erlangen, Germany
Oliver Zolk
Affiliation:
zolk@pharmakologie.uni-erlangen.de, University of Erlangen, Institute of Experimental and Clinical Pharmacology and Toxicology, Erlangen, Germany
Falk Schrödl
Affiliation:
Falk.Schroedl@anatomie1.med.uni-erlangen.de, Paracelsus Medical University, Deptartments of Anatomy/Ophthalmology, Salzburg, Austria
Carola Kryschi
Affiliation:
kryschi@chemie.uni-erlangen.de, University of Erlangen, Physical Chemistry I, Erlangen, Germany
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Abstract

Biocompatible, water-soluble, green luminescent silicon quantum dots (SiQDs) were developed as transfection tool for small interfering RNA (siRNA). The research goal was to down-regulate via the RNA interference (RNAi) mechanism the P-glycoprotein expression of the multidrug resistant gene 1 (MDR1) in a human colon carcinoma cell line (Caco-2). The internalization of 2-vinylpyridine terminated SiQDs (2-vipySiQDs) by Caco-2 cells as observed in confocal laser scanning microscopy imaging studies occurs via endocytosis. Experiments employing agarose gel electrophoresis revealed that 2-vipySiQD-siRNA complexes are formed through electrostatic interactions. The release of siRNA in the cytosol with subsequently RNAi induced down-regulation of the P-glycoprotein translation was verified by detecting a reduced ABCB1 mRNA level in transfected Caco-2 cells employing real-time PCR. Additional evidence for successful ABCB1 gene silencing was obtained by measuring a significant decrease of the P-glycoprotein transporter efficiency for the fluorescent substrate Rhodamine 123 (Rh123).

Keywords

biomedicalluminescencenanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1257: Symposium O – Multifunctional Nanoparticle Systems-Coupled Behavior and Applications , 2010 , 1257-O07-06
Copyright
Copyright © Materials Research Society 2010

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