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Adhesion of Lipid Membranes Mediated by Electrostatic and Specific Interactions

Published online by Cambridge University Press:  15 February 2011

Christian W. Maier ,
Almuth Behrisch ,
Annette Kloboucek  and
Rudolf Merkel
Christian W. Maier
Affiliation:
Lehrstuhl für Biophysik, E22, Technische Universität München, James-Franck-Str. 1, D-85748 Garching, Germany
Almuth Behrisch
Affiliation:
Lehrstuhl für Biophysik, E22, Technische Universität München, James-Franck-Str. 1, D-85748 Garching, Germany
Annette Kloboucek
Affiliation:
Lehrstuhl für Biophysik, E22, Technische Universität München, James-Franck-Str. 1, D-85748 Garching, Germany
Rudolf Merkel
Affiliation:
Lehrstuhl für Biophysik, E22, Technische Universität München, James-Franck-Str. 1, D-85748 Garching, Germany
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Abstract

We used the micropipet aspiration technique for a study of biomembrane adhesion. Adhesion was caused by contact site A, a highly specific cell adhesion molecule, reconstituted in lipid vesicles of DOPC with 5 %(mol/mol) DOPE-PEG2000. We found adhesion and subsequent receptor aggregation in the contact zone. Additionally, electrostatic modulation of membrane adhesion was studied. Whereas addition of the negatively charged lipid SOPS to the lecithin (SOPC) host membrane suppressed adhesion due to electrostatic repulsion, a positively charged lipid (DOTAP) was surprisingly ineffective. This might be due to either phase separation of the mixture or DOTAP changing other membrane properties as bending stiffness and the Hamaker constant.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 489: Symposium K – Materials Science of the Cell , 1997 , 107
Copyright
Copyright © Materials Research Society 1998

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