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Anti-Tumor Chloroquine-Gold Nanocomposites and their Binding Interaction with Bovine Serum Albumin: Biophysical and Biochemical Aspects of Protein Binding

Published online by Cambridge University Press:  21 March 2011

Prachi Joshi ,
Soumyananda Chakraborti ,
Jaime E. Ramirez-Vick ,
Z. A. Ansari ,
Virendra Shanker ,
Pinak Chakrabarti  and
Surinder P. Singh
Prachi Joshi
Affiliation:
National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India. Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi-110025, India.
Soumyananda Chakraborti
Affiliation:
Department of Biochemistry, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata-700054, India.
Jaime E. Ramirez-Vick
Affiliation:
Engineering Science and Materials Department, University of Puerto Rico, Mayaguez, PR 00680, USA.
Z. A. Ansari
Affiliation:
Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi-110025, India.
Virendra Shanker
Affiliation:
National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
Pinak Chakrabarti*
Affiliation:
Department of Biochemistry, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata-700054, India.
Surinder P. Singh*
Affiliation:
National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India. Engineering Science and Materials Department, University of Puerto Rico, Mayaguez, PR 00680, USA.
*
*Address for correspondence: pinak@boseinst.ernet.in; surinder.singh@upr.edu
*Address for correspondence: pinak@boseinst.ernet.in; surinder.singh@upr.edu
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Abstract

We have conjugated chloroquine onto nano-sized, thiol-stabilized gold nanoparticles by using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) / N-hydroxysulfosuccinimide (NHS) chemistry. The formation of gold nanoparticles was confirmed using optical spectra for characteristic surface plasmon band; the average size of gold nanoparticles was found to be 5-7 nm from electron microscopy measurements. The anti-tumor activity of prepared nanocomposite, vis-à-vis chloroquine itself, had been demonstrated using MCF-7 breast cancer cell line. To determine the binding affinity of gold-chloroquine nanocomposites to transport proteins present in blood serum, we studied the binding interaction of gold-chloroquine to bovine serum albumin (BSA), the most abundant plasma protein. The binding was studied by using isothermal titration calorimetry and fluorescence spectroscopy and was analyzed in terms of binding constant, entropy and enthalpy change. The gold-chloroquine nanocomposites were found to interact efficiently with BSA and fluorescence quenching experiments involving Trp212 suggests that the nanocomposites bind at site I of BSA.

Keywords

nanostructurebiomedicalsurface chemistry
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1316: Symposium QQ –Nanofunctional Materials, Nanostructures and Nanodevices for Biomedical Applications II , 2011 , mrsf10-1316-qq09-29
Copyright
Copyright © Materials Research Society 2011

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Footnotes

$

Both the authors have equal contribution.

References

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