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Biocompatibility Assessment of SiC Surfaces After Functionalization with Self Assembled Organic Monolayers

Published online by Cambridge University Press:  31 January 2011

Alexandra Oliveros ,
Sebastian J. Schoell ,
Christopher Frewin ,
Marco Hoeb ,
Martin Stutzmann ,
Ian D. Sharp  and
Stephen E. Saddow
Alexandra Oliveros
Affiliation:
amolive4@mail.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Sebastian J. Schoell
Affiliation:
sebastian.schoell@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Christopher Frewin
Affiliation:
hlodyn676@msn.com, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Marco Hoeb
Affiliation:
Marco.Hoeb@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Martin Stutzmann
Affiliation:
Stutz@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Ian D. Sharp
Affiliation:
ian.sharp@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Stephen E. Saddow
Affiliation:
saddow@eng.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
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Abstract

The biocompatibility of 6H-SiC (0001) surfaces was increased by more than a factor of six through the covalent grafting of NH2 terminated self-assembled monolayers (SAM) using APDEMS and APTES molecules. Surface functionalization began with a hydroxyl, OH, surface termination. The study included two NH2 terminated surfaces obtained through silanization with APDEMS (aminopropyldiethoxymethylsilane) and APTES (aminopropyltriethoxysilane) molecules (hydrophilic surfaces) and a CH3 terminated surface produced via alkylation with 1-octadecene (hydrophobic surface). H4 human neuroglioma and PC12 rat pheochromocytoma cells were seeded on the functionalized surfaces and the cell morphology was evaluated with atomic force microscopy (AFM). In addition, 96 hour MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were employed to evaluate the cell viability on the SAM modified samples. The biocompatibility was enhanced with a 2 fold (171-240%) increase with 1-octadecene, 3-6 fold (320-670%) increase with APDEMS and 5-8 fold (476-850%) increase with APTES with respect to untreated 6H-SiC surfaces.

Keywords

biomaterialself-assemblyscanning probe microscopy (SPM)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1235: Symposium RR – Engineering Biomaterials for Regenerative Medicine , 2009 , 1235-RR03-43
Copyright
Copyright © Materials Research Society 2010

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References

1 Williams, D.F., On the mechanisms of biocompatibility. Biomaterials, 29, 2941–2953 (2008).Google Scholar
2 Miyamoto, S., Akiyama, SK, Yamada, KM. Synergistic roles form receptor occupancy and aggregation in integrin transmembrane function. Science, 267, 883–885 (1995).Google Scholar
3 Vahlberg, C., Yazdi, G.R., Petoral, R.M. Jr. , Syväjärvi, M., Uvdal, K., Spetz, A. Lloyd, Yakimova, R., Khranovsky, V.. Surface engineering of functional materials for Biosensors Sensors, 4, 504–507 (2005).Google Scholar
4 Saddow, S. E. and Agrawal, A., Editors, “Advances in Silicon Carbide Processing and Applications”, © 2004 Artech House ISBN 1-58053-740-5Google Scholar
5 Coletti, C, Jaroszeski, MJ, Pallaoro, A, Hoff, AM, Iannotta, S, Saddow, SE. Biocompatibility and wettability of crystalline SiC and Si surfaces. IEEE EMBC Proceedings 5849-5852 (2007).Google Scholar
6 Frewin, C. L., Jaroszeski, M., Weeber, E., Muffly, K.E., Kumar, A., Peters, M., Oliveros, A., and Saddow, S.E., Atomic force microscopy analysis of central nervous system cell morphology on silicon carbide and diamond substrates Journal of Molecular Recognition 22, 380–388 (2009).Google Scholar
7 Wakamatsu, Y, Zhao, X, Jin, C, Day, N, Shibahara, M, Nomura, N, Nakahara, T, Murata, T, KK, Yokoyama. 2001. Mannosylerythritol lipid induces characteristics of neuronal differentiation in PC12 cells through an ERK-related signal cascade. Eur. J. Biochem. 268: 374–383.Google Scholar
8 Stutzmann, M., Garrido, J. A., Eickhoff, M., and Brandt, M. S., Direct biofunctionalization of semiconductors: A survey, Physica Status Solidi, 203, 3424–3437 (2006).Google Scholar
9 Faucheux, N., Schweiss, R., Lutzow, K., Werner, C., Groth, T., Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies, Biomaterials 25, 2721–2730 (2004)Google Scholar
10 Rosso, Michel, Arafat, Ahmed, Schroen, Karin, Giesbers, Marcel, Roper, Christopher S., Maboudian, Roya, and Zuilhof, Han, Covalent Attachment of Organic Monolayers to Silicon Carbide Surfaces, Langmuir, 24, 4007–4012 (2008).Google Scholar
11 Coletti, C., Frewin, C. L., Hoff, A. M. and Saddow, S. E., Electronic passivation of 3C-SiC (001) via hydrogen treatment, Electrochemical and Solid-State Letters, 11, H285–H287 (2008)Google Scholar
12 Hoeb, M., Sharp, I.D., Schoell, S.J., Alvarez, C. Diaz, Stutzmann, M., and Brandt, M.S., Organic and Bio-organic Functionalization of Hydroxylated Silicon Carbide Surfaces, in preparation (2009).Google Scholar
13 Schoell, S. J., Hoeb, M., Sharp, I. D., Steins, W., Eickhoff, M., Stutzmann, M., and Brandt, M. S., Functionalization of 6H-SiC surfaces with organosilanes, Appl. Phys. Lett., 92, 153301–1 (2008).Google Scholar
14 Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol Methods 65, 55–63 (1983).Google Scholar
15 Sharp, I. D., Schoell, S. J., Hoeb, M., Brandt, M. S., and Stutzmann, M., Electronic properties of self-assembled alkyl monolayers on Ge surfaces, Appl. Phys. Lett., 92, 223306–1, (2008).Google Scholar
16 Lowa, S.P., Williams, K.A., Canhamc, L.T., Voelckera, N.H., Evaluation of mammalian cell adhesion on surface-modified porous silicon, Biomaterials, 27, 4538–4546 (2006).Google Scholar