Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-29T06:52:38.106Z Has data issue: false hasContentIssue false
  • English
  • Français

Bio-inspired Design of Modular Multi-domain Polymers for Advanced Biomaterials

Published online by Cambridge University Press:  01 February 2011

Jason T. Roland ,
Dora Guzman ,
Jane Bai  and
Zhibin Guan
Jason T. Roland
Affiliation:
Department of Chemistry, University of California, 516 Rowland Hall, Irvine, CA 92697-2025
Dora Guzman
Affiliation:
Department of Chemistry, University of California, 516 Rowland Hall, Irvine, CA 92697-2025
Jane Bai
Affiliation:
Department of Chemistry, University of California, 516 Rowland Hall, Irvine, CA 92697-2025
Zhibin Guan
Affiliation:
Department of Chemistry, University of California, 516 Rowland Hall, Irvine, CA 92697-2025
Get access

Abstract

Native load-bearing proteins, such as the muscle protein titin, exhibit a remarkable degree of combined toughness, strength, and elasticity which have yet to be matched by synthetic materials.Single molecule nanomechanical studies on titin and other modular proteins suggest that these exceptional properties arise from a modular elongation mechanism. The sequential unfolding allows modular biopolymers to sustain a large force over the whole extension of the chain, which makes the polymer strong, along with a large area under the force-extension curve, making it tough as well. In addition, when the external force is removed, the unfolded domains of modular proteins will refold automatically, making them elastic. Inspired by nature, one research effort in my group is aimed at designing synthetic macromolecules that form high order structures by programming non-covalent interactions into polymer chain. The goal is to achieve synthetic biomaterials with combined strength, toughness and elasticity. Three classes of well-defined modular polymers have been synthesized in our laboratory: (1) using quadruple hydrogen-bonding motif 2-ureidon-4-pyrimidone (Upy) to direct the formation loops along a polymer chain (J. Am. Chem. Soc.2004, 126, 2058); (2) using a peptidomimetic beta-sheet based double-closed loop (DCL) as module (J. Am. Chem. Soc.2004, 126, 14328); and (3) an engineered protein G domain III as module. Single molecule force-extension experiments revealed the sequential unfolding of the loops or domains as these modular polymers are stretched, resulting in sawtooth-patterned curves similar to those seen in titin and other biopolymers. In this paper, we will discuss our designs, syntheses and single-molecule studies of polymers having modular domain structures.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 873: Symposium K – Biological and Bio-Inspired Materials and Devices , 2005 , K4.9
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Hearle, J. W. S., Fundamentals of Structure and Mechanics; Polymers and Their Properties, Vol 1; Halstead Press: New York, 1982.Google Scholar
2 Booth, C., Price, C., Eds. Polymer Properties; Comprehensive Polymer Science: The Synthesis, Characterization, Reactions, and Applications of Polymers, Vol. 2; Pergamon Press: New York, 1989.Google Scholar
3 Law, R.; Carl, P.; Harper, S.; Dalhaimer, P.; Speicher, D. W.; Discher, D. E. Biophys. J. 2003, 84, 533–544.Google Scholar
4 Rief, M.; Gautel, M.; Oesterhelt, F.; Fernandez, J. M.; Gaub, H. E. Science 1997, 276, 1109–1112.Google Scholar
5 Kellermayer, M. S. Z.; Smith, S. B.; Granzier, H. L.; Bustamante, C. Science 1997, 276, 1112–1116.Google Scholar
6 Li, H.; Oberhauser, A. F.; Fowler, S. B.; Clarke, J.; Fernandez, J. M. Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 6527–6531.Google Scholar
7 Smith, B. L.; Schaffer, T. E.; Viani, M.; Thompson, J. B.; Frederick, N. A.; Kindt, J.; Belcher, A.; Stucky, G. D.; Morse, D. E.; Hansma, P. K. Nature 1999, 299, 761–763.Google Scholar
8 Guan, Z.; Roland, J. T.; Bai, J. Z.; Ma, X. S.; McIntire, T. M.; Nguyen, M. J. Am. Chem. Soc. 2004, 126, 2059–2065.Google Scholar
9 Roland, J. T.; Guan, Z. J. Am. Chem. Soc. 2004, 126, 14328.Google Scholar
10 Sijbesma, R. P.; Beijer, F. H.; Brunsveld, L.; Folmer, B. J. B.; Hirschberg, J. H. K., K.; Lnag, R. F. M.; Lowe, J. K. L. Meijer, E. W. Science 1997, 278, 1601–1604.Google Scholar
11 Gong, B.; Yan, Y.; Zeng, H.; Skrzypczak-Jankunn, E.; Kim, Y. W.; Zhu, J.; Ickes, H. J. Am. Chem. Soc. 1999, 121, 5607–5608.Google Scholar