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Biosurfactant Production Using Mixed Cultures Under Non-Aseptic Conditions

Published online by Cambridge University Press:  15 February 2011

C. Vipulanandan ,
G. L. Ghurye  and
R. C. Willson
C. Vipulanandan
Affiliation:
Department of Civil and Environmental Engineering, University of Houston, Houston, Texas 77204, USA
G. L. Ghurye
Affiliation:
Department of Civil and Environmental Engineering, University of Houston, Houston, Texas 77204, USA
R. C. Willson
Affiliation:
Department of Chemical Engineering, University of Houston, Houston, Texas 77204-4792, USA
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Abstract

Surfactants increase the accessibility of adsorbed hydrocarbons and mobilize immiscible petroleum hydrocarbons for treatment. Biosurfactants have the advantage of biodegradability and non-toxicity over their synthetic counterparts, and can be produced from renewable sources. In this study the production of biosurfactant from molasses was investigated in continuously stirred batch reactors. The effects of substrate concentration, yeast extract and peptone on biomass accumulation and biosurfactant production were investigated. Biosurfactant production was quantified by surface tension reduction and critical micelle dilution (CMD). Biosurfactant production was directly correlated with biomass production, and was improved with the addition of yeast extract. Centrifugation of the whole broth reduced surface tension. The performance of the biosurfactant produced from molasses under non-aseptic condition is comparable to other published results.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 344: Symposium I – Materials and Processes for Environmental Protection , 1994 , 315
Copyright
Copyright © Materials Research Society 1994

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