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Chitosan-alginate Hybrid Scaffolds for in Vitro Bone Tissue Regeneration

Published online by Cambridge University Press:  01 February 2011

Zhensheng Li ,
Hassna R. Ramay ,
Kip D. Hauch  and
Miqin Zhang
Zhensheng Li
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
Hassna R. Ramay
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
Kip D. Hauch
Affiliation:
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Miqin Zhang
Affiliation:
Department of Materials Science & Engineering, University of Washington, Seattle, 302L Roberts Hall, WA 98195-2120, USA
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Abstract

This paper reports the development of a biodegradable porous scaffold made from naturally derived chitosan and alginate polymers for bone tissue engineering. The scaffold has a 3-D interconnected porous structure and was fabricated through thermally induced phase separation. The mechanical test showed that the scaffold has compressive strength of 0.46 ± 0.02 MPa — about 4 times that of the pure chitosan scaffold. The cell-material interaction study indicated that osteoblast cells seeded on the chitosan-alginate scaffold attach and proliferate well. The mineral deposition occurred after 3 days of culture and formed large chunks after 7 days. The chitosan-alginate scaffold was also found to have desirable swelling property and was structurally stable in solutions of different pH. The chitosan-alginate scaffold can be prepared from solutions of neutral pH allowing growth factors to be incorporated homogeneously into the scaffold for sustained release. This research demonstrated a technique by which a polymer-based biodegradable scaffold can be made to have high porosity up to 92% and excellent mechanical and biological properties.

Keywords

chitosanalginatescaffoldbone tissuebiodegradable
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 873: Symposium K – Biological and Bio-Inspired Materials and Devices , 2005 , K8.2
Copyright
Copyright © Materials Research Society 2005

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References

1 Jarcho, M., Calcium phosphate ceramics as hard tissue prosthetics. Clin Orthop, 1981(157): p. 259–78.Google Scholar
2 Hench, L.L. and Wilson, J., Surface-active biomaterials. Science, 1984. 226(4675): p. 630–6.Google Scholar
3 Yang, S., et al., The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Eng, 2001. 7(6): p. 679–89.Google Scholar
4 Jolles, P., , R.A.A.M.E., Chitin and Chitinases. 1999, Birkhauser, Basel.Google Scholar
5 Madihally, S.V. and Matthew, H.W., Porous chitosan scaffolds for tissue engineering. Biomaterials, 1999. 20(12): p. 1133–42.Google Scholar
6 Thanoo, B.C., Sunny, M.C., and Jayakrishnan, A., Cross-linked chitosan microspheres: preparation and evaluation as a matrix for the controlled release of pharmaceuticals. J Pharm Pharmacol, 1992. 44(4): p. 283–6.Google Scholar
7 Park, Y.J., et al., Platelet derived growth factor releasing chitosan sponge for periodontal bone regeneration. Biomaterials, 2000. 21(2): p. 153–9.Google Scholar
8 Klokkevold, P.R., et al., steogenesis enhanced by chitosan (poly-N-acetyl glucosaminoglycan) in vitro. J Periodontol, 1996. 67(11): p. 1170–5.Google Scholar
9 Muzzarelli, C. and Muzzarelli, R.A., Natural and artificial chitosan-inorganic composites. J Inorg Biochem, 2002. 92(2): p. 89–94.Google Scholar
10 Shanmugasundaram, N., et al., Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells. Biomaterials, 2001. 22(14): p. 1943–51.Google Scholar
11 Zhang, S., Emerging biological materials through molecular self-assembly. Biotechnol Adv, 2002. 20(5-6): p. 321–39.Google Scholar
12 Zhao, F., et al., Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds. Biomaterials, 2002. 23(15): p. 3227–34.Google Scholar
13 Zhang, Y. and Zhang, M., Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants. J Biomed Mater Res, 2002. 61(1): p. 1–8.Google Scholar
14 Zhang, Y. and Zhang, M., Calcium phosphate/chitosan composite scaffolds for controlled in vitro antibiotic drug release. J Biomed Mater Res, 2002. 62(3): p. 378–86.Google Scholar
15 Li, Z., et al., Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials, 2005. 26(18): p. 3919–28.Google Scholar
16 Porter, B.D., et al., Mechanical properties of a biodegradable bone regeneration scaffold. J Biomech Eng, 2000. 122(3): p. 286–8.Google Scholar
17 Chu, T.M., et al., Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures. Biomaterials, 2002. 23(5): p. 1283–93.Google Scholar
18 Thomson, R.C., et al., Fabrication of biodegradable polymer scaffolds to engineer trabecular bone. J Biomater Sci Polym Ed, 1995. 7(1): p. 23–38.Google Scholar
19 Vachoud, L., Zydowicz, N., and Domard, A., Physicochemical behaviour of chitin gels. Carbohydr Res, 2000. 326(4): p. 295–304.Google Scholar
20 Khalid, M.N., et al., Water state characterization, swelling behavior, thermal and mechanical properties of chitosan based networks. Eur J Pharm Sci, 2002. 15(5): p. 425–32.Google Scholar