Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-10T08:42:59.813Z Has data issue: false hasContentIssue false
  • English
  • Français

Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies

Published online by Cambridge University Press:  31 January 2011

Lijuan Zhang ,
Young Wook chun  and
Thomas J Webster
Lijuan Zhang
Affiliation:
lijuan_zhang@brown.edu, Brown University, chemistry, Providence, Rhode Island, United States
Young Wook chun
Affiliation:
young_wook_chun@brown.edu, Brown University, engineering, providence, Rhode Island, United States
Thomas J Webster
Affiliation:
thomas.webster@scholarone.com, Brown University, engineering, providence, Rhode Island, United States
Get access

Abstract

PLGA (poly-lactic-co-glycolic acid) has been widely used as a biomaterial in regenerative medicine due to its biocompatibility and biodegradability properties. Previous studies have shown that cells (such as bladder smooth muscle cells, chondrocytes, osteoblasts, and vascular smooth muscle cells) respond differently to nano-structured PLGA surfaces (such as those with surface features less than 100 nm in at least one dimension) compared to nano-smooth surfaces. The purpose of the present in vitro research was to prepare PLGA films with various nanometer surface features and determine, for the first time, whether lung cancer epithelial cells respond differently to such topographies. Poly(dimethylsiloxane) (PDMS) molds prepared by placing PDMS onto various polystyrene monolayers and two solution evaporation methods were used to create nanometer surface features on PLGA. The intended spherical surface nano-topographies on PLGA with RMS values of 2.23, 5.03, 5.42 and 36.90 nm were formed, while PLGA surfaces with RMS values of 0.62 and 2.23 nm were obtained by different solution evaporation methods. Most importantly, lung cancer epithelial cells adhered less on the PLGA surfaces with an RMS value of 0.62, 2.23 and 5.42 nm after 4 hours of culture compared to any other PLGA surface created here. After three days, PLGA surfaces with an RMS value of 0.62 nm had much lower cell density than any other sample. In this manner, PLGA with specific nanometer surface features may inhibit lung cancer cell density which may provide for an important biomaterial for the treatment of lung cancer for a wide range of applications (from drug delivery to regenerative medicine).

Keywords

biomaterialnanostructurepolymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1209: Symposia P/YY – Business and Safety Issues in the Commercialization of Nanotechnology , 2009 , 1209-YY07-08
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Dalby, M.J., et al., Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography. Biomaterials, 2004. 25(23): p. 5415–5422.Google Scholar
2 Nomura, S., et al., Cell culture on nanopillar sheet: Study of HeLa cells on nanopillar sheet. Japanese Journal of Applied Physics Part 2-Letters & Express Letters, 2005. 44(37-41): p. L1184–L1186.Google Scholar
3 Turner, S., et al., Cell attachment on silicon nanostructures. Journal of Vacuum Science & Technology B, 1997. 15(6): p. 2848–2854.Google Scholar
4 Charest, J.L., Garcia, A.J. and King, W.P. Myoblast alignment and differentiation on cell culture substrates with microscale topography and model chemistries. Biomaterials, 2007. 28 (13): p. 2202–2210.Google Scholar
5 Wood, M.A., Wilkinson, C.D. and Curtis, A.S. The effects of colloidal nanotopography on initial fibroblast adhesion and morphology. IEEE Trans Nanobioscience, 2006. 5(1): p. 20–31.Google Scholar
6 Dalby, M.J., Pasqui, D., and Affrossman, S., Cell response to nano-islands produced by polymer demixing: a brief review. IEE Proc Nanobiotechnol, 2004. 151(2): p. 53–61.Google Scholar
7 Yim, E.K., et al., Nanopattern-induced changes in morphology and motility of smooth muscle cells. Biomaterials, 2005. 26(26): p. 5405–13.Google Scholar
8 Zhu, B., et al., Alignment of osteoblast-like cells and cell-produced collagen matrix induced by nanogrooves. Tissue Eng, 2005. 11(5-6): p. 825–34.Google Scholar
9 Coletti, D., et al., Static magnetic fields enhance skeletal muscle differentiation in vitro by improving myoblast alignment. Cytometry Part A, 2007. 71A(10): p. 846–856.Google Scholar
10 Cornfield, J., et al., Smoking and lung cancer: recent evidence and a discussion of some questions. International Journal of Epidemiology, 2009. 38(5): p. 1175–1191.Google Scholar
11 Carpenter, J., Khang, D., and Webster, T.J. Nanometer polymer surface features: the influence on surface energy, protein adsorption and endothelial cell adhesion. Nanotechnology, 2008. 19(50): p. -.Google Scholar
12 Liu, H. and Webster, T.J. Nanomedicine for implants: A review of studies and necessary experimental tools. Biomaterials, 2007. 28(2): p. 354–369.Google Scholar
13 Choi, C.H., et al., Cell interaction with three-dimensional sharp-tip nanotopography. Biomaterials, 2007. 28(9): p. 1672–1679.Google Scholar
14 Lee, J.Y., et al., The control of cell adhesion and viability by zinc oxide nanorods. Biomaterials, 2008. 29(27): p. 3743–3749.Google Scholar