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Detection of Cryptosporidium parvum in antibody-doped gels

Published online by Cambridge University Press:  10 February 2011

E. Hong ,
E. Bescher ,
L. Garcia  and
J. D. Mackenzie
E. Hong
Affiliation:
UCLA Department of Materials Science and Engineering, Los Angeles, CA 90095
E. Bescher
Affiliation:
UCLA Department of Materials Science and Engineering, Los Angeles, CA 90095
L. Garcia
Affiliation:
UCLA Department of Microbiology, Los Angeles, CA 90095
J. D. Mackenzie
Affiliation:
UCLA Department of Materials Science and Engineering, Los Angeles, CA 90095
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Abstract

Doping porous oxides with organic functionalities has become increasingly useful for the detection of many types of compounds and molecules. So far the detection of microorganisms by this process has not been firmly established. There is, however, a need to develop easy to use, sensitive devices for the detection of microorganisms in potable water supplies. For example, the need for a sensor that can detect a pathogenic parasite called Cryptosporidium parvum has emerged in the United States. In this report, we describe the production of such a device via the sol-gel method. The sol-gel sensor can detect trace amounts of Cryptosporidium parvum. The advantages of the porous oxide matrix lead to high sensitivity. The sensor is easy to use and inexpensive. The presence of Cryptosporidium parvum is indicated by a color change in an otherwise colorless gel. Absorbance intensities for the enzyme-linked antibody used in the study correlated appropriately to oocyst concentration in the solution.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 435: Symposium V – Better Ceramics Through Chemistry VII , 1996 , 449
Copyright
Copyright © Materials Research Society 1996

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