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Detection of Lectins using Glyco-Functionalized Nanosensors

Published online by Cambridge University Press:  12 July 2012

Yanan Chen ,
Harindra Vedala ,
Gregg P. Kotchey ,
Aymeric Audfray ,
Samy Cecioni ,
Anne Imberty ,
Sébastien Vidal  and
Alexander Star
Yanan Chen
Affiliation:
Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260, USA
Harindra Vedala
Affiliation:
Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260, USA
Gregg P. Kotchey
Affiliation:
Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260, USA
Aymeric Audfray
Affiliation:
CERMAV - CNRS, affiliated with Université Joseph Fourier and ICMG, BP 53, 38041, Grenoble, France
Samy Cecioni
Affiliation:
CERMAV - CNRS, affiliated with Université Joseph Fourier and ICMG, BP 53, 38041, Grenoble, France Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Laboratoire de Chimie Organique 2 – Glycochimie, UMR 5246, CNRS, Université Claude Bernard Lyon 1, 43 Boulevard du 11 Novembre 1918, F-69622, Villeurbanne, France
Anne Imberty
Affiliation:
CERMAV - CNRS, affiliated with Université Joseph Fourier and ICMG, BP 53, 38041, Grenoble, France
Sébastien Vidal
Affiliation:
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Laboratoire de Chimie Organique 2 – Glycochimie, UMR 5246, CNRS, Université Claude Bernard Lyon 1, 43 Boulevard du 11 Novembre 1918, F-69622, Villeurbanne, France
Alexander Star
Affiliation:
Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260, USA
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Abstract

We have used single-walled carbon nanotube field-effect transistor (SWNT-FET) and chemically converted graphene field-effect transistor (CCG-FET) devices to probe the interactions between carbohydrates and their recognition lectins. Porphyrin- and pyrene-based glycoconjugates were used as receptor molecules and the target lectins were two bacterial lectins that present different carbohydrate preference, namely PA-IL, PA-IIL from Pseudomonas aeruginosa and a plant lectin Concanavalin A. The specific binding between lectin and carbohydrate can be transduced to the change in FET device conductance. An initial study with SWNT-FET noncovalently functionalized with porphyrin-based glycoconjugates showed both good selectivity and sensitivity. To compare SWNT and CCG performance, pyrene- and porphyrin-based glycoconjugates were functionalized noncovalently on the surface of CCG-FET and SWNT-FET devices, which were then treated with non-specific and specific lectins. The responses were compared and rationalized using computer-aided models of carbon nanostructure/glycoconjugate interactions. Fluorescence microscopy, atomic force microscopy, UV-vis-NIR spectroscopy and Isothermal titration microcalorimetry (ITC) measurements were used to confirm the electrical results.

Keywords

sensornanostructuremicroelectronics
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1451: Symposium DD/EE – Nanocarbon Materials and Devices , 2012 , pp. 191 - 196
Copyright
Copyright © Materials Research Society 2012

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