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The Development of Silicon Carbide Based Electrode Devices for Central Nervous System Biomedical Implants

Published online by Cambridge University Press:  31 January 2011

Christopher Frewin ,
Alexandra Oliveros ,
Christopher Locke ,
Irina Filonova ,
Justin Rogers ,
Edwin Weeber  and
Stephen E. Saddow
Christopher Frewin
Affiliation:
clfrewin@mail.usf.eduhlodyn676@msn.com, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Alexandra Oliveros
Affiliation:
amolive4@mail.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Christopher Locke
Affiliation:
clocke@mail.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Irina Filonova
Affiliation:
amerishka@gmail.com, University of South Florida, Molecular Pharmacology and Physiology Department, Tampa, Florida, United States
Justin Rogers
Affiliation:
jrogers3@health.usf.edu, University of South Florida, Molecular Pharmacology and Physiology Department, Tampa, Florida, United States
Edwin Weeber
Affiliation:
eweeber@health.usf.edu, University of South Florida, Florida Center of Excellence for Biomolecular Identification and Targeted Therapeutics (FCoE-BITT), Tampa, Florida, United States
Stephen E. Saddow
Affiliation:
saddow@ieee.org, University of South Florida, Electrical Engineering, Tampa, Florida, United States
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Abstract

Brain machine interface (BMI) technology has been demonstrated to be a therapeutic solution for assisting people suffering from damage to the central nervous system (CNS), but BMI devices using implantable neural prosthetics have experienced difficulties in that they are recognized by glial cells as being foreign material, which leads to an immune response cascade process called gliosis. One material, cubic silicon carbide (3C-SiC), may provide an excellent solution for the generation of an implantable neural prosthetic interface component of a BMI system. We have recently reported on the biocompatibility of 3C-SiC with immortalized cells, and have extended this work by demonstrating neural cell action potential instigation via an electrode type device. Biocompatibility assessment of 3C-SiC was accomplished using in vitro methodology. 96 hour MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays were performed to determine neural cell viability. Atomic force microscopy (AFM) was used to quantify attached cell morphology and determine lamellipodia/ filopodia interaction with the surface of the semiconductor. It was seen that neurons show excellent viability, cell morphology, and good lamellipodia/ filopodia permissiveness when interacting with 3C-SiC. A neuronal activation device (NAD), based on the planar Michigan microelectrode probe, was constructed from 3C-SiC with the goal of activating an action potential within a neuron. In order to illicit an action potential, neurons were seeded on the NAD device and then they were subjected to a biphasic square pulse signal. Successful action potential activation was recorded through the use of Rhod-2, a Ca2+ sensitive fluorescent dye. Based on these results, 3C-SiC may be an excellent material platform for neural prosthetics.

Keywords

biomaterialbiomedicaldevices
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1236: Symposium SS – Biosurfaces and Biointerfaces , 2009 , 1236-SS01-02
Copyright
Copyright © Materials Research Society 2010

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