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Disposable organic fluorescence biosensor for water pollution monitoring.

Published online by Cambridge University Press:  07 July 2011

Florent Lefèvre ,
Luping Yu ,
Vamsy Chodavarapu ,
Philippe Juneau  and
Ricardo Izquierdo
Florent Lefèvre
Affiliation:
Department of Computer Science, Resmiq, NanoQAM, Université du Québec à Montréal, Montréal, QC, H3C 3P8, CANADA.
Luping Yu
Affiliation:
Department of Chemistry and the James Franck Institute, The University of Chicago, E. 57th Street, Chicago, Illinois 60637, U.S.A.
Vamsy Chodavarapu
Affiliation:
Department of Electrical and Computer Engineering, Resmiq, McGill University, 3480 University St., Room 642, Montréal, QC, H3A 2A7, CANADA.
Philippe Juneau
Affiliation:
Department of biology, TOXEN, Université du Québec à Montréal, Montréal, QC, H3C 3P8, CANADA
Ricardo Izquierdo
Affiliation:
Department of Computer Science, Resmiq, NanoQAM, Université du Québec à Montréal, Montréal, QC, H3C 3P8, CANADA.
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Abstract

We report the first disposable fluorescent biosensor based on algae, with an organic light emitting diode and an organic photodetector (OPD) miniaturized into a microfluidic chip. A DPVBi OLED was used as the excitation source, while a blend of PTB3/PCBM was used for the organic photodetector. The fluorescence biosensor is integrated in a microfluidic chip made from polymeric materials such as (poly)dimethylsiloxane (PDMS), which is transparent, biocompatible and can easily be processed by conventional lithography. The complete detector is designed to detect Chlamydomonas reinhardtii green algae fluorescence in the microfluidic chamber. Algal chlorophyll fluorescence is a physiological parameter routinely used to measure the photochemical efficiency of PSII. This measurement is a reliable and non-invasive method to determine the toxicity of pollutants like herbicides and metals.

Keywords

sensormicroelectronicsoptoelectronic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1358: Symposium MM – Organic Bioelectronics and Photonics for Sensing and Regulation , 2011 , mrss11-1358-mm05-03
Copyright
Copyright © Materials Research Society 2011

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References

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