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Effective temperature inside living cells

Published online by Cambridge University Press:  31 January 2011

Claire Wilhelm
Claire Wilhelm*
Affiliation:
claire.wilhelm@univ-paris-diderot.fr, Laboratoire MSC. UMR 7057 CNRS & Université Paris Diderot, PARIS, United States
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Abstract

The combination of active and passive microrheology using magnetic probes engulfed inside living cells demonstrates the violation of the fluctuation dissipation theorem in cells. It is proposed to quantify the deviation from the in equilibrium situation with an effective temperature. Each magnetic probe then serves as a local thermometer within the cells. The response of pairs of magnetic beads of two diameters (1 and 2.8 μm) to an oscillating magnetic field is analyzed to measure the viscoelastic complex modulus in the beads environment (active measurement). The spontaneous motion of the beads is tracked to compute their mean square displacements (passive measurement). The effective temperature is derived using an extension of the fluctuation dissipation theorem.

Keywords

biologicaldiffusionmagnetic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1227: Symposium JJ – Multiscale Dynamics in Confining Systems , 2009 , 1227-JJ05-03
Copyright
Copyright © Materials Research Society 2010

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