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The Effects of Chemical Functionalization vs. Biological Functionalization on Nanoparticle Binding Affinity

Published online by Cambridge University Press:  01 February 2011

Jason J Benkoski ,
Julia J Patrone ,
James Crookston ,
Huong Le  and
Jennifer Sample
Jason J Benkoski
Affiliation:
jason.benkoski@jhuapl.edu, The Johns Hopkins University Applied Physics Laboratory, Milton Eisenhower Research Center, 11100 Johns Hopkins Rd, MS 9-110, Laurel, MD, 20723, United States, 443-778-5140, 443-778-5850
Julia J Patrone
Affiliation:
julia.patrone@jhuapl.edu, The Johns Hopkins University Applied Physics Laboratory, Milton Eisenhower Research Center, 11100 Johns Hopkins Rd, Laurel, MD, 20723, United States
James Crookston
Affiliation:
james.crookston@jhuapl.edu, The Johns Hopkins University Applied Physics Laboratory, Milton Eisenhower Research Center, 11100 Johns Hopkins Rd, Laurel, MD, 20723, United States
Huong Le
Affiliation:
huong.le@jhuapl.edu, The Johns Hopkins University Applied Physics Laboratory, Milton Eisenhower Research Center, 11100 Johns Hopkins Rd, Laurel, MD, 20723, United States
Jennifer Sample
Affiliation:
jennifer.sample@jhuapl.edu, The Johns Hopkins University Applied Physics Laboratory, Milton Eisenhower Research Center, 11100 Johns Hopkins Rd, Laurel, MD, 20723, United States
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Abstract

Due to their small size, high diffusivity, and chemically active surfaces, nanoparticles share much in common with water-soluble proteins. These similarities have generated great interest in using biofunctional nanoparticles as a route to deliver targeted therapeutics. Already nanoparticles have found applications in the hyperthermia of tumors, personal care lotions, and tissue scaffolding. Our study focuses on the targeting of such nanoparticles to specific biological sites. It therefore seeks to identify the factors that control the migration and capture of nanoparticles within living systems. In particular, we examine the affinity of anti-collagen coated magnetite nanoparticles for collagen IV-coated surfaces under flow stress. The studies are performed within microfluidic devices that are designed to mimic various fluid flow patterns within the body. We find, in this case, a large background signal due to nonspecific binding. Further examination shows a correlation between chemical functionality (e.g., surface charge, hydrophilicity) that suggests that a balanced approach between biological functionality and chemical functionality may reduce the background from nonspecific binding to acceptable levels.

Keywords

nanoscalebiomaterialsurface chemistry
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1064: Symposium PP – Quantum-Dot and Nanoparticle Bioconjugates–Tools for Sensing and Biomedical Imaging , 2007 , 1064-PP03-04
Copyright
Copyright © Materials Research Society 2008

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References

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