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Effects on Hardness and Elastic Modulus for DSS-8 Peptide Treatment on Remineralization of Human Dental Tissues

Published online by Cambridge University Press:  15 March 2011

Chia-Chan Hsu ,
Hsiu-Ying Chung ,
Elizabeth Marie Hagerman ,
Wenyuan Shi ,
Jenn-Ming Yang  and
Ben Wu
Chia-Chan Hsu
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
Hsiu-Ying Chung
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A. Department of Materials Science and Engineering, Feng Chia University, Taichung 407, Taiwan, Republic of China
Elizabeth Marie Hagerman
Affiliation:
Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
Wenyuan Shi
Affiliation:
School of Dentistry, University of California, Los Angeles, CA 90095, U.S.A.
Jenn-Ming Yang
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
Ben Wu
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A. Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
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Abstract

Dental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in enamel remineralization. Human enamel specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Enamel nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using the grazing incidence X-ray diffraction (GIXD), variable pressure scanning electron microscopy (VPSEM), and atomic force microscopy (AFM), respectively. Nanoindentation results show that the DSS-8 remineralization effectively improves the mechanical and elastic properties for demineralized enamel.

Keywords

EnamelPeptideNanoindentationRemineralization
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1132: Symposium Z – Mechanics of Biological and Biomedical Materials , 2008 , 1132-Z09-05
Copyright
Copyright © Materials Research Society 2009

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References

1. Landis, WJ, Song, MJ, Leith, A, McEwen, L, McEwen, BF. Mineral and organic matrix interaction in normally calcifying tendon visualized in three dimensions by high-voltage electron microscopic tomography and graphic image reconstruction. J Struct Biol 1993;110:39–54.Google Scholar
2. Nanci, A. Ten cate's oral histology. St. Louis: Mosby Elsevier;2003.Google Scholar
3. Suga, S, Watabe, N. Hard tissue mineralization and demineralization. Tokyo: Springer; 1992.Google Scholar
4. Cuy, JL, Mann, AB, Livi, KJ, Teaford, MF, Weihs, TP. Nanoindentation mapping of the mechanical properties of human molar tooth enamel. Arch Oral Biol 2002; 47:281–91.Google Scholar
5. Stetler, WGS, Veis, A. Bovine dentin phosphophoryn: calcium ion binding properties of a high molecular weight preparation. Calcif Tissue Int 1987; 40(2):97–102.Google Scholar
6. George, A, Bannon, L, Sabsay, B, Dillon, JW, Malone, J, Veis, A, Jenkins, NA, Gilbert, DJ, Copeland, NG. The carboxyl-terminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J Biol Chem 1996; 271 (51): 32869–73.Google Scholar
7. He, G, Dahl, T, Veis, A, Geroge, A. Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1. Nat Mater 2003;2(8):552–8.Google Scholar
8. Sikes, CS, Yeung, ML, Wheeler, AP. In surface reactive peptides and polymers: discovery and commercialization. Washington: ACS;1991.Google Scholar
9. Qiu, SR, Wierzbicki, A, Orme, CA, Cody, AM, Hoyer, JR, Nancollas, GH, Zepeda, S, Yoreo, JJD. Molecular modulation of calcium oxalate crystallization by osteopontin and citrate. Proc Natl Acad Sci 2004;101(7):1811–5.Google Scholar
10. Bigi, A, Boanini, E, Rubini, K, Gazzano, M. Hydroxyapatite nanocrystals modified with acidic amino acids. Eur J Inorg Chem 2006;23: 4821–6.Google Scholar
11. Papanearchou, NI, Leventouri, T, Kis, AC, Hotiu, A, Anderson, IM. Effect of simulated body fluid on the microstructure of ferrimagnetic bioglass-ceramics. Mater Res Soc Symp Proc 2005;839:371–6.Google Scholar
12. Kokubo, T, Takadama, H. How useful is SBF in predicting in vivo bone bioactivity. Biomaterials 2006:27:2907.Google Scholar
13. Murphy, WL, Monney, DJ. Bioinspired growth of crystalline carbonate apatite on biodegradable polymer substrata. J Am Chem Soc 2002;124(9):1910–7.Google Scholar
14. Oliver, WC, Pharr, GM. Review: Measurement of hardness and elastic modulus by instrumented indentation: Advances in understanding and refinements to methodology. J Mater Res 2004;19(1):3–20.Google Scholar
15. Zhou, J, Hsiung, LL. Biomolecular origin of the rate-dependent deformation of prismatic enamel. Appl Phys Lett 2006;89:51904.Google Scholar
16. He, LH, Swain, MV. Influence of environment on the mechanical behavior of mature human enamel. Biomaterials 2007;28:4512–20Google Scholar
17. JCPDS, No. 00-001-1008. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar
18. JCPDS, No. 00-035-0180. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar
19. JCPDS, No. 00-004-0697. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar