Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-13T06:00:28.179Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of Biological Responses of UMR-106 Cells to Porous PHBV Matrix

Published online by Cambridge University Press:  01 February 2011

Dharmaraj Raghavan ,
Hui Liu  and
John Stubbs III
Dharmaraj Raghavan
Affiliation:
draghavan@howard.edu, Howard, Chemistry, 525 College Street, NW, Washington DC, DC, 20059, United States
Hui Liu
Affiliation:
liuhui4546@yahoo.com, Howard University, Washington DC, DC, 20059, United States
John Stubbs III
Affiliation:
istubbs@howard.edu, Howard University, Washington DC, DC, 20059, United States
Get access

Abstract

This paper compared the results of osteoblast-like UMR-106 cells response to 3-D biodegradable porous with nonporous PHBV (poly (3-hydroxybutyrate-co-3-hydroxyvalerate) films. Nonporous PHBV films were prepared by solvent casting and evaporation. Porous PHBV films were prepared by solute leaching of salt/PHBV cast film. Sieved sodium chloride (∼150 μm) was used to create a matrix with high porosity and then leached out by the solvent. Thermo gravimetric analysis showed that over 99% of the salts were leached out by the solvent. Osteoblast-like UMR-106 cells were seeded onto the nonporous and porous PHBV films, respectively. After 6 and 10 days of incubation, growth rate of UMR-106 cells on porous film was higher than on nonporous film. The viable cell proliferation on the porous and nonporous PHBV film was quantified by (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) (MTS) assay. The results of this study suggest that the UMR-106 cells proliferate more on the porous PHBV matrix and porous PHBV films are a promising material for bone tissue engineering applications.

Keywords

solvent castingbiomaterialporosity
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D15-06
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Mezzenga, R, Ruokolainen, J, Fredrickson, GH, Kramer, EJ, Moses, D, Heeger, AJ, et al. Templating organic semiconductors via self-assembly of polymer colloids. Science (Washington, DC, United States) 2003;299(5614):1872–1874.Google Scholar
2. Yang, H, Lopina, ST. Extended release of a novel antidepressant, venlafaxine, based on anionic polyamidoamine dendrimers and poly(ethylene glycol)-containing semi interpenetrating networks. Journal of Biomedical Materials Research, Part A 2005;72A(1):107–114.Google Scholar
3. Koese, GT, Korkusuz, F, Oezkul, A, Soysal, Y, Oezdemir, T, Yildiz, C, et al. Tissue engineered cartilage on collagen and PHBV matrices. Biomaterials 2005;26(25):5187–5197.Google Scholar
4. Chen G-Q, Wu, Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials 2005;26(33):6565–6578.Google Scholar
5. Carina, Eldser SKaA-CA. Effect of abiotic factors on the degradation of poly(3- hydroxybutyrate-co-3-hydroxyvalerate) in simulated and natural composting environments. Polymer Degradation and Stability 1999 May;64(2):177–183.Google Scholar
6. Tesema, Y, Raghavan, D, Stubbs, J, III. Bone cell viability on collagen immobilized poly(3-hydroxybutrate-co-3-hydroxyvalerate) membrane: Effect of surface chemistry. Journal of Applied Polymer Science 2004;93(5):2445–2453.Google Scholar
7. Gunaratne, LMWK, Shanks, RA. Multiple melting behavior of poly(3-hydroxybutyrateco-hydroxyvalerate) using step- scan DSC. European Polymer Journal 2005;41(12):2980–2988.Google Scholar
8. Sarazin, P, Virgilio, N, Favis, BD. Influence of the porous morphology on the in vitro degradation and mechanical properties of poly(L-lactide) disks. Journal of Applied Polymer Science 2006;100(2):1039–1047.Google Scholar
9. Smith, E, Yang, J, McGann, L, Sebald, W, Uludag, H. RGD-grafted thermoreversible polymers to facilitate attachment of BMP-2 responsive C2C12 cells. Biomaterials 2005 Dec;26(35):7329–7338.Google Scholar
10. Liu, H, Tesema, Y, Stubbs, J III, Raghavan, D. In vitro degradation of porous PHBV film. Polymer Preprints (American Chemical Society, Division of Polymer Chemistry) 2005;46(2):1246–1247.Google Scholar