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Evaporative Deposition of Bacteria from a Sessile Drop: Effects of Suspension Aging

Published online by Cambridge University Press:  01 February 2011

Kyle Fisher Baughman ,
Raina M. Maier  and
Joan E Curry
Kyle Fisher Baughman
Affiliation:
kfbaughman@gmail.combaughman@u.arizona.edu
Raina M. Maier
Affiliation:
rmaier@ag.arizona.edu, University of Arizona, Soil, Water and Environmental Science, Tucson, Arizona, United States
Joan E Curry
Affiliation:
curry@ag.arizona.edu, University of Arizona, Soil, Water and Environmental Science, 1177 E 4th St, Tucson, Arizona, 85721, United States
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Abstract

In this work, we report on the evaporative deposition of bacteria from a drying aqueous drop on mica as a function of the bacterial suspension age. For sufficiently aged bacterial suspensions, residues are small and more filled-in than residues formed from fresh suspensions on similarly aged mica. In addition, the interior deposition pattern transitions from a cellular film characteristic of fresh suspensions to a cracked carpet pattern for aged suspensions. Suspension aging related changes in the residues are attributed to accumulation of organic materials such as DNA, RNA, proteins, and other bacterial components in the suspension. The aging process is also observed to be at least partially dependent on ventilation of the suspension during aging.

Keywords

biologicalfluidicsbiomaterial
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1273: Symposium MM – Evaporative Self Assembly of Polymers, Nanoparticles, and DNA , 2010 , 1273-MM03-04
Copyright
Copyright © Materials Research Society 2010

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References

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