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Fatigue Microdamage in Bovine Cortical Bone Imaged by Micro-Computed Tomography Using a Barium Sulfate Contrast Agent

Published online by Cambridge University Press:  26 February 2011

Huijie Leng ,
Xiang Wang ,
Glen L Niebur  and
Ryan K Roeder
Huijie Leng
Affiliation:
hleng@nd.edu, University of Notre Dame, 365 Fitzpatrick Hall, Notre Dame, IN, 46556, United States, 5746314381
Xiang Wang
Affiliation:
xwang10@nd.edu, United States
Glen L Niebur
Affiliation:
gniebur@nd.edu, United States
Ryan K Roeder
Affiliation:
rroeder@nd.edu, United States
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Abstract

Accumulation of microdamage during fatigue can lead to increased fracture susceptibility in bone. Current techniques for imaging microdamage in bone are inherently destructive and two-dimensional. A non-destructive, three-dimensional technique is needed to measure the spatial density of microdamage accumulation. Therefore, the objective of this study was to image microdamage accumulation in cortical bone during fatigue using micro-computed tomography (micro-CT) with a barium sulfate (BaSO4) contrast agent. Bovine cortical bone beams were loaded under four-point bending fatigue. Two symmetric notches were machined on the tensile surface in order to generate damage at the stress concentrations during loading. Specimens were loaded to a specified number of cycles or until one notch fractured, such that the other notch exhibited accumulated microdamage just prior to fracture. Microdamage ahead of the notch was stained by precipitation of BaSO4 and imaged using micro-CT. Reconstructed images showed a distinct region of bright voxels around the notch tip or along propagating cracks due to the presence of BaSO4, which was verified by backscattered electron imaging and energy dispersive spectroscopy. The stained region exhibited a characteristic kidney shape perpendicular to the notch tip, which was correlated to principal strain contours calculated by finite element analysis. The area of stained regions was positively correlated with the number of loading cycles.

Keywords

bonefatiguemicrostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 898: Symposium L – Mechanical Behavior of Biological and Biomimetic Materials , 2005 , 0898-L09-04
Copyright
Copyright © Materials Research Society 2006

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