Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2025-01-05T02:58:46.692Z Has data issue: false hasContentIssue false
  • English
  • Français

Generating an Intracellular Diffusion Map by Two-Photon Fluorescence Correlation Spectroscopy

Published online by Cambridge University Press:  15 February 2011

P. T. C. So ,
K. Duggar ,
K. M. Berland  and
E. Gratton
P. T. C. So
Affiliation:
ME Dept., MIT, Cambridge, MA 02139, ptso@mit.edu
K. Duggar
Affiliation:
ME Dept., MIT, Cambridge, MA 02139, ptso@mit.edu
K. M. Berland
Affiliation:
TJ Watson Research Center, IBM, Yorktown Heights, NY
E. Gratton
Affiliation:
LFD, Dept. of Physics, UIUC, Urbana, IL 61801
Get access

Abstract

A new technique for determining cellular viscosity has been developed based on two-photon excitation and fluorescence correlation spectroscopy. We have applied this method to map intracellular diffusion coefficient of small molecules at selected cytoplasmic locations of mouse fibroblast cells. The cytoplasmic diffusion rate is one tenth of that in an aqueous environment.

Keywords

two-photonFCSfluorescence correlation spectroscopycytoplasmic diffusion
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 489: Symposium K – Materials Science of the Cell , 1997 , 187
Copyright
Copyright © Materials Research Society 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Elson, E. L. and Magde, D., 1974, Fluorescence correlation spectroscopy I. Conceptual basis and theory, Biopolymers, 13, 1–27.Google Scholar
2. Elson, E. L., Magde, D., and Webb, W. W., 1974, Fluorescence correlation spectroscopy II. An experimental realization, Biopolymers, 13, 29–61.Google Scholar
3. Thompson, N.L., 1991, Fluorescence correlation spectroscopy in Topics in fluorescence spectrosopy, Vol. 1: Techniques, Lakowicz, J. R., Ed., (New York: Plenum).Google Scholar
4. Qian, H and Elson, E. L., App. Opt., 30, pp.1185–1195 (1991).Google Scholar
5. Berland, K. M., So, P. T. C. and Gratton, E., Biophys. J, 68, pp. 694–701 (1995).Google Scholar
6. Berland, K. M., So, P. T. C., Chen, Y., Mantulin, W. W., and Gratton, E., Biophys. J. 71, pp. 410–420 (1996).Google Scholar
7. Eigne, M. and Rigler, R., Proc. Nat'l. Acad. Sci. USA, 91, pp. 5740–5747 (1994).Google Scholar
8. Kinjo, M. and Rigler, R., Nucleic-acid Res., 23, pp. 1795–1799 (1995).Google Scholar
9. Denk, W., Strickler, J. H., and Webb, W. W., Science, 248, pp. 73–76 (1990).Google Scholar