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Grating Coupled Surface Plasmon Resonance Enhanced Fluorescence and Its Application for Cell Observation

Published online by Cambridge University Press:  31 January 2011

Xiaoqiang Cui ,
Keiko Tawa  and
Junji Nishii
Xiaoqiang Cui
Affiliation:
xiaoqiang-cui@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering, Osaka, Japan
Keiko Tawa
Affiliation:
tawa-keiko@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering, Osaka, Japan
Junji Nishii
Affiliation:
junji.nishii@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering, Osaka, Japan
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Abstract

One-dimensional grating with 400 nm pitch was fabricated on a SiO2 glass surface. The grating was applied for detection of fluorescence excited by the electric field of the grating-coupled surface plasmon resonance and highly sensitive observation of fluorescence image by optical microscope. 40-times of fluorescence enhancement was obtained under optimal condition after our systematically investigating depth and duty ratio dependent behavior.

Keywords

nanostructurebiomedicalphotochemical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1208: Symposium O – , 2009 , 1208-O18-12
Copyright
Copyright © Materials Research Society 2010

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