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Highly Sensitive Surface Plasmon Resonance Sensor on Nanoscale Bioactive Surfaces for Specific Detection of Tri-Nitro Toluene

Published online by Cambridge University Press:  01 February 2011

Praveen Singh ,
Takeshi Onodera ,
Kiyoshi Matsumoto ,
Norio Miura  and
Kiyoshi Toko
Praveen Singh
Affiliation:
psingh67@yahoo.com, Kyushu University, Department of Electronics, 744 Motooka, Nishi-ku, R.No.459,W2, Fukuoka, 819-0395, Japan, 81-92-8023762, 81-92-8023770
Takeshi Onodera
Affiliation:
onodera@ed.kyushu-u.ac.jp, Kyushu University, Department of Electronics, Graduate school of Information Science and Electrical Engineeting, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan
Kiyoshi Matsumoto
Affiliation:
kmatsu@agr.kyushu-u.ac.jp, Kyushu University, Graduate School of Agriculture, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan
Norio Miura
Affiliation:
miura@astec.kyushu-u.ac.jp, Kyushu University, Art, Science and Technology Centre for Cooperative Research, Kasuga-shi, Fukuoka, 816-8580, Japan
Kiyoshi Toko
Affiliation:
toko@ed.kyushu-u.ac.jp, Kyushu University, Department of Electronics, Graduate School of Information Science and Electrical Engineering, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan
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Abstract

A nano-scale biosensor chip surface was fabricated using dinitro-phenylated key hole limpet (DNP-KLH) protein conjugate as ligand supported by underlying 11-amino 1-undecanethiol hydrochloride(AUT) self assembled monolayer (SAM) and bis sulfosuccinimidyl suberate(BS3) as crosslinker. Bioactive thin films were fabricated over gold chip via layer-by-layer self assembly methods. Biomolecular interaction between substrate specific anti-TNT antibody and DNP-KLH conjugate surface was monitored through surface plasmon resonance based optical sensor. The quantitation of tri-nitro toluene(TNT) on this bioactive surface was done using the solution based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.14 ng/ml(140 ppt) for TNT molecule. The detection limit of surface plasmon resonance(SPR) biosensor was further enhanced by using goat anti mouse antibody to the 0.002 ng/ml for TNT analyte. This TNT specific biosensor holds the promise to be one of most sensitive sensor surface under indirect competitive assay format. A short injection (12 sec) of 10 mM Glycine-HCl solution was found adequate for regeneration of DNP-KLH surface for repeated use.

Keywords

nanoscaleopticalbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 951: Symposium E – Nanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection , 2006 , 0951-E07-12
Copyright
Copyright © Materials Research Society 2007

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