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Influence of DSS-8 on the remineralisation of Dentine

Published online by Cambridge University Press:  31 January 2011

Chia-Chan Hsu ,
Elizabeth Marie Hagerman ,
Hsiu-Ying Chung ,
Wenyuan Shi ,
Jenn-Ming Yang  and
Ben Wu
Chia-Chan Hsu
Affiliation:
richardhsu@ucla.edu, University of California Los Angeles, Materials Science and Engineering, Los Angeles, California, United States
Elizabeth Marie Hagerman
Affiliation:
sodium0303@hotmail.com, University of California Los Angeles, Bioengineering, Los Angeles, California, United States
Hsiu-Ying Chung
Affiliation:
richardhsu0303@yahoo.com, Feng Chia University, Materials Science and Engineering, Taichung, Taiwan, Taiwan, Province of China
Wenyuan Shi
Affiliation:
sodium0303@yahoo.com.tw, University of California, Los Angeles, School of Dentistry, Los Angeles, California, United States
Jenn-Ming Yang
Affiliation:
chiachan@seas.ucla.edu, United States
Ben Wu
Affiliation:
pqucla@yahoo.com, United States
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Abstract

Dental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in dentin remineralization. Human dentin specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Dentin nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using grazing incidence X-ray diffraction, variable pressure scanning electron microscopy, and atomic force microscopy, respectively. Nanoindentation results show that DSS-8 remineralization effectively improves the mechanical and elastic properties of native dentin. Moreover, the hardness and elastic modulus for the DSS-8 treated dentin were significantly higher than surfaces remineralized without DSS-8.

Keywords

nano-indentationscanning electron microscopy (SEM)x-ray diffraction (XRD)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1187: Symposium KK – Structure-Property Relationships in Biomineralized and Biomimetic Composites , 2009 , 1187-KK05-34
Copyright
Copyright © Materials Research Society 2009

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References

[1] GWJ, Marshall, SJ, Marshall, JH, Kinney, Balooch, M. The dentin substrate: structure and properties related to bonding. J Dent 1997;25:441–58.Google Scholar
[2] Linde, A, Lundgren, T. From serum to the mineral phase. The role of the odontoblast in calcium transport and mineral formation. Int J Dev Biol 1995;39:213–22.Google Scholar
[3] WT, Butler, Ritchie, H. The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol 1995;39:169–79.Google Scholar
[4] WGS, Stetler, Veis, A. Bovine dentin phosphophoryn: calcium ion binding properties of a high molecular weight preparation. Calcif Tissue Int 1987;40(2):97–102.Google Scholar
[5] He, G, Dahl, T, Veis, A, Geroge, A. Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1. Nat Mater 2003;2(8):552–8.Google Scholar
[6] CS, Sikes, ML, Yeung, AP, Wheeler. In surface reactive peptides and polymers: discovery and commercialization. Washington: ACS;1991.Google Scholar
[7] SR, Qiu, Wierzbicki, A, CA, Orme, AM, Cody, JR, Hoyer, GH, Nancollas, Zepeda, S, JJD, Yoreo. Molecular modulation of calcium oxalate crystallization by osteopontin and citrate. Proc Natl Acad Sci 2004;101(7):1811–5.Google Scholar
[8] Bigi, A, Boanini, E, Rubini, K, Gazzano, M. Hydroxyapatite nanocrystals modified with acidic amino acids. Eur J Inorg Chem 2006;23: 4821–6.Google Scholar
[9] NI, Papanearchou, Leventouri, T, AC, Kis, Hotiu, A, IM, Anderson. Effect of simulated body fluid on the microstructure of ferrimagnetic bioglass-ceramics. Mater Res Soc Symp Proc 2005;839:371–6.Google Scholar
[10] Kokubo, T, Takadama, H. How useful is SBF in predicting in vivo bone bioactivity. Biomaterials 2006:27:2907.10.1016/j.biomaterials.2006.01.017Google Scholar
[11] WL, Murphy, DJ, Monney. Bioinspired growth of crystalline carbonate apatite on biodegradable polymer substrata. J Am Chem Soc 2002;124(9):1910–7.Google Scholar
[12] JCPDS No. 01–074-0566. International Center for Diffraction Data: Newton Square, PA 2003.Google Scholar
[13] JCPDS No. 00–035-0180. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar