Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-14T18:38:37.706Z Has data issue: false hasContentIssue false
  • English
  • Français

Layer-by-layer printing of cells and its application to tissue engineering

Published online by Cambridge University Press:  01 February 2011

Priya Kesari ,
Tao Xu  and
Thomas Boland
Priya Kesari
Affiliation:
Department of Bioengineering, Clemson University, Clemson, South Carolina 29634
Tao Xu
Affiliation:
Department of Bioengineering, Clemson University, Clemson, South Carolina 29634
Thomas Boland
Affiliation:
Department of Bioengineering, Clemson University, Clemson, South Carolina 29634
Get access

Abstract

Tissues and organs exhibit distinct shapes and functions nurtured by vascular connectivity. In order to mimic and examine these intricate structure-function relationships, it is necessary to develop efficient strategies for assembling tissue-like constructs. Many of the top-down fabrication techniques used to build microelectromechanical systems, including photolithography, are attractive due to the similar feature sizes, but are not suitable for delicate biological systems or aqueous environments. A layer-by layer approach has been proposed by us to pattern functional cell structures in three dimensions. Freeform cell structures are created by the inkjet method, in which cells are entrapped within hydrogels and crosslinked on demand. The cells are viable, functional and show potential for cell maturation as exemplified by the diversion of hematopoietic stem cells into multiple cell types. These results show promise for many tissue engineering applications.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA4.5
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Fuchs, JR, Nasseri, BA, Vacanti, JP. Tissue engineering: a 21st century solution to surgical reconstruction. Annals of Thoracic Surgery 2001; 72(2):577–591.Google Scholar
2. Langer, R, Vacanti, JP. Tissue engineering. Science 1993; 260(5110):920–926.Google Scholar
3. Hutmacher, DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000; 21(24):2529–2543.Google Scholar
4. Yang, S, Leong, KF, Du, Z, Chua, CK. The design of scaffolds for use in tissue engineering. Part II.Rapid prototyping techniques. Tissue Engineering 2002; 8(1):1–11.Google Scholar
5. Vozzi, G, Flaim, C, Ahluwalia, A, Bhatia, S. Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition. Biomaterials 2003; 24(14):2533–2540.Google Scholar
6. Landers, R, Hubner, U, Schmelzeisen, R, Mulhaupt, R. Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. Biomaterials 2002; 23(23):4437–4447.Google Scholar
7. Sachlos, E, Czernuszka, JT. Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. European Cells & Materials 2003; 5:29–39.Google Scholar
8. Sachlos, E, Reis, N, Ainsley, C, Derby, B, Czernuszka, JT. Novel collagen scaffolds with predefined internal morphology made by solid freeform fabrication. Biomaterials 2003; 24(8):1487–1497.Google Scholar
9. Whitesides, GM, Grzybowski, B. Self-assembly at all scales. Science 2002; 295(5564):2418–2421.Google Scholar
10. Salazar-Ciudad, I, Jernvall, J, Newman, SA. Mechanisms of pattern formation in development and evolution. Development 2003; 130(10):2027–2037.Google Scholar
11. Wilson, WC Jr, Boland, T. Cell and organ printing 1: protein and cell printers. Anatomical Record 2003; 272A(2):491–496.Google Scholar
12. Mironov, V, Boland, T, Trusk, T, Forgacs, G, Markwald, RR. Organ printing: computer-aided jet-based 3D tissue engineering. Trends in Biotechnology 2003; 21(4):157–161.Google Scholar
13. Boland, T, Mironov, V, Gutowska, A, Roth, EA, Markwald, RR. Cell and organ printing 2: fusion of cell aggregates in three-dimensional gels. Anatomical Record 2003; 272A(2):497–502.Google Scholar
14. Sun, W, Lal, P. Recent development on computer aided tissue engineering—a review. Computer Methods & Programs in Biomedicine 2002; 67(2):85–103.Google Scholar
15. Foty, RA, Pfleger, CM, Forgacs, G, Steinberg, MS. Surface tensions of embryonic tissues predict their mutual envelopment behavior. Development 1996; 122(5):1611–1620.Google Scholar
16. Lim, F, Sun, AM. Microencapsulated islets as bioartificial endocrine pancreas. Science 1980; 210(4472):908–910.Google Scholar
17. Griffith, LG, Naughton, G. Tissue engineering--current challenges and expanding opportunities. Science 2002; 295(5557):1009–1014.Google Scholar
18. Cabrita, GJ, Ferreira, BS, da Silva, CL, Goncalves, R, Almeida-Porada, G, Cabral, JM. Hematopoietic stem cells: from the bone to the bioreactor. Trends in Biotechnology 2003; 21(5):233–240.Google Scholar
19. Stock, UA, Vacanti, JP. Tissue engineering: current state and prospects. Annual Review of Medicine 2001; 52:443–451.Google Scholar
20. Xu, T, Jin, J, Gregory, C, Hickman, JJ, Boland, T. Inkjet printing of viable mammalian cells. Biomaterials 2005; 26:93–99.Google Scholar