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Microfabricated, Wireless, Magnetoelastic Micro-Particles for the Detection of Bacillus Anthracis Spores

Published online by Cambridge University Press:  01 February 2011

Jiehui Wan ,
Michael L. Johnson ,
Huihua Shu ,
Valery A. Petrenko  and
Bryan A. Chin
Jiehui Wan
Affiliation:
wanjieh@auburn.edu, Auburn University, Material Engineering, 275 Wilmore Laboratory, Auburn University, AL, 36849, United States, 334-844-3126, 334-844-3400
Michael L. Johnson
Affiliation:
johnsml@auburn.edu, Auburn University, Material Engineering, 275 Wilmore Laboratory, Auburn, AL, 36849, United States
Huihua Shu
Affiliation:
shuhuih@auburn.edu, Auburn University, Electrical Engineering, Auburn, AL, 36849, United States
Valery A. Petrenko
Affiliation:
petreva@vetmed.auburn.edu, Auburn University, Department of Pathobiology, Auburn, AL, 36849, United States
Bryan A. Chin
Affiliation:
bchin@eng.auburn.edu, Auburn University, Material Engineering, 275 Wilmore Laboratory, Auburn, AL, 36849, United States
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Abstract

Magnetoelastic resonance biosensors were fabricated by immobilizing a bio-molecular recognition element onto the surface of Fe79B21 magnetoelastic particles (MEP). These sensors can be measured wirelessly and remotely for both in-air and in-liquid bacteria detection. Filamentous bacterio-phage that was selected specifically for the detection of Bacillus anthracis spores was employed as the biomolecular recognition element and immobilized onto the MEPs' surfaces. Attachment of the spores to the sensor surface due to specific phage-spore binding results in a shift in the resonance frequency of the biosensor. Insitu measurement of the resonance frequency of biosensors of 5×100×500 microns were used to determine the sensor response as a function of spore concentrations of 103 to 108 cfu/ml. Specificity of the sensor was evaluated by conducting tests using a mixture of Bacillus anthracis Sterne strain, Bacillus cereus and Bacillus megaterium spores.

Keywords

biomaterialacousticsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 951: Symposium E – Nanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection , 2006 , 0951-E06-31
Copyright
Copyright © Materials Research Society 2007

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