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Miniaturization of Immunoassays Using Optical Detection with Integrated Amorphous Silicon Photodiodes

Published online by Cambridge University Press:  31 January 2011

Ana Teresa Pereira ,
Virginia Chu ,
Duarte M. F. Prazeres  and
Joao P Conde
Ana Teresa Pereira
Affiliation:
apereira@inesc-mn.pt, INESC Microsistemas e Nanotecnologias and IN- Institute of Nanoscience and Nanotechnology, Lisbon, Portugal
Virginia Chu
Affiliation:
vchu@inesc-mn.pt, INESC Microsistemas e Nanotecnologias and IN- Institute of Nanoscience and Nanotechnology, Lisbon, Portugal
Duarte M. F. Prazeres
Affiliation:
miguelprazeres@inesc-mn.pt, Centro de Engenharia Biológica e Química, IBB – Institute of Biotechnology and Bioengineering, Instituto Superior Técnico, Lisbon, Portugal
Joao P Conde
Affiliation:
joao.conde@ist.utl.pt, INESC Microsistemas e Nanotecnologias and IN- Institute of Nanoscience and Nanotechnology, Lisbon, Portugal
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Abstract

Immunoassays are currently the main analytical technique for quantification of a wide range of analytes of clinical, medical, biotechnological, and environmental significance with high sensitivity and specificity. Miniaturization of immunoassays is achieved using microfluidics coupled with integrated optical detection of the antibody-antigen molecular recognition reaction using thin-film amorphous silicon (a-Si:H) photodiodes. The detection system used consists of an a-Si:H photodiode aligned with a polydimethylsiloxane (PDMS) microchannel. An enzymatic reaction taking place in the microchannel yields a product which is a light-absorbent molecule and hence can be optically detected by the integrated photodiode. Specific antigen-antibody reaction was detected and distinguished from the non-specific reaction.

Keywords

fluidicsbiologicalabsorption
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1191: Symposium OO – Materials and Strategies for Lab-on-a-Chip–Biological Analysis, Cell-Material Interfaces and Fluidic Assembly , 2009 , 1191-OO08-04
Copyright
Copyright © Materials Research Society 2009

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References

[1] Street, R. A., Hydrogenated amorphous silicon: Cambridge University Press, 1991.10.1017/CBO9780511525247Google Scholar
[2] Pimentel, A. C. et al. , “Detection of chemiluminescence using an amorphous silicon photodiode,” Ieee Sensors Journal, vol. 7, pp. 415–416, Mar-Apr 2007.10.1109/JSEN.2006.890144Google Scholar
[3] Gouvea, A. et al. , “Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate,” Sensors and Actuators B-Chemical, vol. 135, pp. 102–107, Dec 2008.10.1016/j.snb.2008.07.030Google Scholar
[4] Pereira, A. T. et al. , “Chemiluminescent Detection of Horseradish Peroxidase Using an Integrated Amorphous Silicon Thin Film Photosensor,” IEEE Sensors Journal, vol. Accepted for publication, 2008.Google Scholar
[5] Bange, A. et al. , “Microfluidic immunosensor systems,” Biosensors & Bioelectronics, vol. 20, pp. 2488–2503, 2005.10.1016/j.bios.2004.10.016Google Scholar
[6] Parsa, H. et al. , “Effect of volume– and time-based constraints on capture of analytes in microfluidic heterogeneous immunoassays,” Lab on a Chip, vol. 8, pp. 2062–2070, 2008.10.1039/b813350fGoogle Scholar
[7] Henares, T. G. et al. , “Current development in microfluidic immunosensing chip,” Analytica Chimica Acta, vol. 611, pp. 17–30, Mar 2008.10.1016/j.aca.2008.01.064Google Scholar