Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-14T05:05:48.515Z Has data issue: false hasContentIssue false
  • English
  • Français

Nano Monitors for Identification of Vulnerable Cardio-Vascular Plaque

Published online by Cambridge University Press:  01 February 2011

Ravikiran Kondama Reddy  and
Shalini Prasad
Ravikiran Kondama Reddy
Affiliation:
ravikk@pdx.edu, Portland State University, Electrical and Computer Engineering, 1600 SW Harrison St, Fourth Avenue Building., Suite 25-02, Porltand, Oregon, 97201, United States
Shalini Prasad
Affiliation:
sprasd@pdx.edu, Portland State University, Electrical and Computer Engineering, 1600 SW Harrison St, Fourth Avenue Building., Suite 25-02, Portland, Oregon, 97201, United States
Get access

Abstract

As our nation's population ages, there will be a substantial demand for surgical services. The best predictor of postoperative outcome is the presence of perioperative ischemia, which is caused by vulnerable coronary plaque rupture. It is not know what makes plaques rupture, but inflammation has been proposed as a unifying etiology. The physiologic perioperative state is one of intense, acute inflammation and thrombosis. This is characterised by the presence of protien- Human Serum C-Reactive Protein( hsCRP) and Myeloperoxidase(MPO). There is a gap in detection capability between gold standard in proteomics detection –Enzyme Linked Immunosorbent Assay (ELISA) assay methods and electrical biosensors.

ELISA based protein biomarker detection in too slow to be applicable in early patient bedside treatment. Electrical biosensors on the other hand may overcome this limitation with their improved sensitivity, specificity and rapid detection. In this application we demonstrate the development of nanomembrane based electrical protein “nano monitor”. This technique overcomes the limitations current “state-of- the- art” methods such as:

• Specificity in detection of multiple markers that characterizes the disease pathogenesis from a single marker to multiple markers.

• Speed of detection from a turnaround time of 12/24 hours to a few minutes.

• Sensitivity of detection from milligram/ml to nanogram/ml.

Keywords

nanoscalebiomedicalsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 915: Symposium R – Nanostructured Materials and Hybrid Composites for Gas Sensors and Biomedical Applications , 2006 , 0915-R06-27
Copyright
Copyright © Materials Research Society 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 DT, Mangano. Perioperative medicine: NHLBI working group deliberations and recommendations. J Cardiothorac Vasc Anesth 2004;18(1):1–6.Google Scholar
2 DT, Mangano, WS, Browner, Hollenberg, M, MJ, London, JF, Tubau, IM, Tateo. Association of perioperative myocardial ischemia with cardiac morbidity and mortality in men undergoing noncardiac surgery. The Study of Perioperative Ischemia Research Group. N Engl J Med 1990;323(26):1781–8.Google Scholar
3 DT, Mangano, EL, Layug, Wallace, A, Tateo, I. Effect of atenolol on mortality and cardiovascular morbidity after noncardiac surgery. Multicenter Study of Perioperative Ischemia Research Group. N Engl J Med 1996;335(23):1713–20.Google Scholar
4 Wallace, A, Layug, B, Tateo, I, et al. Prophylactic atenolol reduces postoperative myocardial ischemia. McSPI Research Group. Anesthesiology 1998;88(1):7–17.Google Scholar
5 Casscells, W, Hathorn, B, David, M, et al. Thermal detection of cellular infiltrates in living atherosclerotic plaques: possible implications for plaque rupture and thrombosis. Lancet 1996;347(9013):1447–51.Google Scholar
6 Danesh, J, Collins, R, Appleby, P, Peto, R. Association of fibrinogen, C-reactive protein, albumin, or leukocyte count with coronary heart disease: meta-analyses of prospective studies. Jama 1998;279(18):1477–82.Google Scholar
7 PM, Ridker, Rifai, N, Rose, L, JE, Buring, NR, Cook. Comparison of C-reactive protein and low-density lipoprotein cholesterol levels in the prediction of first cardiovascular events. N Engl J Med 2002;347(20):1557–65.Google Scholar
8 Blaschke, F, Bruemmer, D, Yin, F, et al. C-reactive protein induces apoptosis in human coronary vascular smooth muscle cells. Circulation 2004;110(5):579–87.Google Scholar
9 Circulation 2004;110:1868–1873.Google Scholar
10 Arterioscler Thromb Vasc Biol 2003;23:2123–2130.Google Scholar
11 Das, B and McGinnis S, P. Novel template-based semiconductor nanostructures and their applications. Appl. Phys. A. Mater. 2000; 71 681­8.Google Scholar
12 Masuda, H and Fukuda, K Ordered metal nanohole arrays made by a two step replication of honeycomb structures of anodic alumina. Science 1995; 268 1466­8.Google Scholar
13 Chen, R.J., Choi, H.C., Bangsaruntip, S., Yenilmez, E., Tang, X., Wang, Q., Chang, Y.L., and Dai, H. An investigation of the mechanisms of electronic sensing of protein adsorption on carbon nanotube devices. J Am Chem Soc. (2004); 126(5), 1563–1568.Google Scholar
14 Cui, Y., Wei, Q.Q., Park, H.K. and Lieber, C.M. Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science 2001;293, 1289–1292.Google Scholar
15 Takhistov, P. Electrochemical synthesis and impedance characterization of nanopatternedbiosensor substrate. Biosens Bioelectron. 2004; 19(11), 1445–1456.Google Scholar
16 Zheng, G., Patolsky, F., Cui, Y., Wang, W.U., and Lieber, C.M. Multiplexed Electrical Detection of Cancer Markers with Nanowire Sensor Arrays Nature, 2005; 23(10), 1294–1301 Google Scholar
17 Hintsche, R., Wallenberger, U, Paeschke, M., and Uhlig, A. Simultaneous analysis of chemical and biochemical species by microelectrode arrays, Proc. Biosensors 1994; Published by New Orleans, S-1-8.Google Scholar
18 Lee, J.H., Yoon, K.H., Hwang, K.S., Park, J., Ahn, S., and Kim, T.S. Label free novel electrical detection using micromachined PZT monolithic thin film cantilever for the detection of C-reactive protein. Biosens. Bioelectron., 2004;20(2), 269–275.Google Scholar
19 Fawcett, W. Dipole­dipole interactions and their role in relaxation processes in polar solvents. Chem. Phys. Lett 1990. 174 (2), 167­175.Google Scholar
20 MacBeath, G. and Schreiber, S.L. Printing proteins as microarrays for high-throughput function determination. Science 2000; 289, 1760–1763.Google Scholar
21 Arenkov, P. et al. Protein microchips: use for immunoassay and enzymatic reactions. Anal. Biochem. 2000; 278, 123–131.Google Scholar
22 Ozin, G.A. Nanochemistry: synthesis in diminishing dimensions. Adv. Mater. 1992; 4 (10), 612­649.Google Scholar
23 , Naghavi et al., Circulation. 2003; Oct 7;108(14):1664–72.Google Scholar