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New biological labels based on functionalized YVO4: Eu nanoparticles

Published online by Cambridge University Press:  01 February 2011

D. Giaume ,
V Buissette ,
K. Lahlil ,
T. Gacoin ,
J.-P. Boilot ,
D. Casanova ,
E. Beaurepaire ,
M.-P. Sauviat ,
A. Mercuri  and
A. Alexandrou
D. Giaume
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
V Buissette
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
K. Lahlil
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
T. Gacoin
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
J.-P. Boilot
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
D. Casanova
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
E. Beaurepaire
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
M.-P. Sauviat
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
A. Mercuri
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
A. Alexandrou
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
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Abstract

Lanthanide-ion doped oxide (YVO4: Eu) nanoparticles were synthesized as aqueous colloids and functionalized by a bioactive silane shell to be used as fluorescent biological labels. Nanoparticles functionalized with guanidinium groups were able to act as artificial toxins which specifically target Na+ channels. They were individually detectable in live cardiac myocytes. Functionalized oxide nanoparticles appear as a new interesting tool, especially attractive for long-term single-molecule tracking due to their photo-stability and long luminescence lifetime.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA6.2
Copyright
Copyright © Materials Research Society 2005

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References

1 Zhang, J.; Campbell, R. E.; Ting, A. Y.; Tsien, R. Y. Nat. Rev. Mol. Cell Biol. 2002, 3, 906–918.Google Scholar
2 Haugland, R. P. Handbook of Fluorescent Probes and Research Products; Molecular Probes: Eugene, OR, 2002.Google Scholar
3 Charpak, S.; Mertz, J.; Beaurepaire, E.; Moreaux, L.; Delaney, K. Proc. Nat. Acad. Sci. USA 2001, 98, 1230–1234.Google Scholar
4 Bruchez, M.; Moronne, M.; Gin, P.; Weiss, S.; Alivisatos, A. P. Science 1998, 281, 2013.Google Scholar
5 Chan, W. C. W.; Nie, S. Science 1998, 281, 2016.Google Scholar
6 Dubertret, B.; Skourides, P.; Norris, D. J.; Noireaux, V.; Brivanlou, A. H.; Libchaber, A. Science 2002, 298, 1759.Google Scholar
7 Dahan, M.; Lévi, S.; Luccardini, C.; Rostaing, P.; Riveau, B.; Triller, A. Science 2003, 302.Google Scholar
8 Wu, X.; Liu, H.; Liu, J.; Haley, K. N.; Tradway, J. A.; Larson, J. P.; Ge, N.; Peale, F.; Bruchez, M. P. Nature Biotech. 2003, 21, 41.Google Scholar
9 Jaiswal, J. K.; Mattousi, H.; Mauro, J. M.; Simon, S. M. Nature Biotech. 2003, 21, 47.Google Scholar
10 Kim, S.; Lim, Y. T.; Soltesz, E. G.; Grand, A. M. D.; Lee, J.; Nakayama, A.; Parker, J. A.; Mihaljevic, T.; Laurence, R. G.; Dor, D. M.; Cohn, L. H.; Bawendi, M. G.; Frangioni, J. V. Nature Biotech. 2004, 22, 93–97.Google Scholar
11 Huignard, A.; Gacoin, T.; Boilot, J.-P. Chem. Mat. 2000, 12, 1090.Google Scholar
12 Huignard, A.; Gacoin, T.; Chaput, F.; Boilot, J.-P. Mat. Res. Soc. Symp. Proc. 2001, 667, G.4.5.1.Google Scholar
13 Narahashi, T. J. Pharmacol. Exp. Ther. 2000, 294, 1.Google Scholar
14 Hille, B. Biophys. J. 1975, 15, 615–619.Google Scholar
15 Kao, C. Y. Ann. N. Y. Acad. Sci. 1986, 479, 52–67.Google Scholar
16 Beaurepaire, E.; Buissette, V.; Sauviat, M.-P.; Giaume, D.; Lahlil, K.; Mercuri, A.; Casanova, D.; Huignard, A.; Martin, J.-L.; Gacoin, T.; Boilot, J.-P.; Alexandrou, A.; Nanoletters 2004, 4(11), 2079–2083.Google Scholar
17 Sauviat, M.-P.; Marquais, M.; Vernoux, J.-P. Toxicon 2002, 40, 1155–1163.Google Scholar
18 Sauviat, M.-P.; Colas, A.; Pages, N. BioMed Central Pharmacology 2002, 2, 15.Google Scholar
19 Sheets, M. F.; Hanck, D. A.; Fozzard, H. A. Circ. Res. 1989, 65, 1462–1465.Google Scholar