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One-step separation of CD20+ cells from whole blood using bacterial magnetic particles displaying protein G

Published online by Cambridge University Press:  01 February 2011

Masayuki Takahashi ,
Tomoko Yoshino ,
Haruko Takeyama  and
Tadashi Matsunaga
Masayuki Takahashi
Affiliation:
masa33@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
Tomoko Yoshino
Affiliation:
y-tomoko@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
Haruko Takeyama
Affiliation:
haruko@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
Tadashi Matsunaga
Affiliation:
tmatsuna@cc.tuat.ac.jp, Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
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Abstract

Magnetic separation of target cells from mixtures, such as peripheral blood and bone marrow, has considerable practical potential in research and medical applications. Among the current cell separation techniques, magnetic cell separation using immunomagnetic particles has been routinely applied and has proven rapidness and simplicity. Magnetospirillum magneticum AMB-1 synthesizes intracellular nano-sized bacterial magnetic particles (BacMPs) that are individually enveloped by a stable lipid bilayer membrane. BacMPs, which exhibit strong ferrimagnetism, can be collected easily with commercially available permanent magnets. In this study, a novel magnetic nanoparticle displaying protein G (protein G-BacMPs) was fabricated, and one-step cell separation for direct cell separation from whole blood was performed using the protein G-BacMPs. B lymphocytes (CD20+ cells), which cover less than 0.3×10−2 % of whole blood cells, were separated with 93% purity using protein G-BacMPs binding with anti-CD20 monoclonal antibodies. The results of this study demonstrate the utility of protein G-BacMPs and the magnetic cell separation approach based on protein G-BacMPs in numerous applications.

Keywords

biomaterialmagneticparticulate
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1094: Symposium DD – From Biological Materials to Biomimetic Material Synthesis , 2008 , 1094-DD07-18
Copyright
Copyright © Materials Research Society 2008

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References

1. Tiwari, A., Punshon, G., Kidane, A., Hamilton, G., and Seifalian, A. M., Cell Biol. Toxicol. 19, 265 (2003).Google Scholar
2. Miltenyi, S., Muller, W., Weichel, W., and Radbruch, A., Cytometry 11, 231 (1990).Google Scholar
3. Kuhara, M., Takeyama, H., Tanaka, T., and Matsunaga, T., Anal. Chem. 76, 6207 (2004).Google Scholar
4. Matsunaga, T., Takahashi, M., Yoshino, T., Kuhara, M., and Takeyama, H., Biochem. Biophys. Res. Commun. 350, 1019 (2006).Google Scholar
5. Blakemore, R. P., Maratea, D., and Wolfe, R. S., J. Bacteriol. 140, 720 (1979).Google Scholar
6. Yoshino, T. and Matsunaga, T., Appl. Environ. Microbiol. 72, 465 (2006).Google Scholar
7. Gronenborn, A. M., Filpula, D. R., Essig, N. Z., Achari, A., Whitlow, M., Wingfield, P. T., and Clore, G. M., Science 253, 657 (1991).Google Scholar
8. Tanaka, T. and Matsunaga, T., Anal. Chem. 72, 3518 (2000).Google Scholar
9. Koehl, U., Beck, O., Esser, R., Seifried, E., Klingebiel, T., Schwabe, D., and Seidl, C., Leukemia 17, 232 (2003).Google Scholar