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PEG-based bioactive hydrogels crosslinked via phosphopantetheinyl transferase

Published online by Cambridge University Press:  01 February 2011

Katarzyna Mosiewicz ,
Kai Johnsson  and
Matthias P. Lutolf
Katarzyna Mosiewicz
Affiliation:
katarzyna.mosiewicz@epfl.ch, Ecole Polytechnique Federal de Lausanne, Laboratory of Stem Cells Bioengineering, Institute of Bioengineering, Lausanne, Switzerland
Kai Johnsson
Affiliation:
kai.johnsson@epfl.ch, Ecole Polytechnique Federal de Lausanne, Laboratory of Protein Engineering, Institute of Bioengineering, Lausanne, Switzerland
Matthias P. Lutolf
Affiliation:
matthias.lutolf@epfl.ch, Ecole Polytechnique Fédérale de Lausanne, Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, Lausanne, Switzerland
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Abstract

State-of-the-art tissue engineering strategies increasingly rely on the performance of bioactive hydrogels formed via cell-friendly crosslinking reactions. Enzymatic reactions possess ideal characteristics for such applications, but they are currently still underexplored in biomaterials design. Here we report the development of hybrid bioactive hydrogels formed via a posttranslational modification reaction using phosphopantetheinyl transferase (PPTase). PPTase was shown to catalyze the covalent crosslinking of CoenzymeA-functionalized poly(ethylene glycol) (PEG) multiarm macromers and recombinantly produced acyl carrier protein (ACP) dimers. Crosslinking kinetics and physicochemical properties of PPTase hydrogels were characterized. Proof-of-principle experiments demonstrate the successful covalent bio-functionalization of gels with a CoA-derivatized cell adhesion peptide. Polymerization of gels in the presence of primary mammalian cells was shown to result in no loss in cell viability compared to a well established, chemically crosslinked gel system.

Keywords

biomimetic (chemical reaction)biomaterialpolymerization
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1272: Symposia KK/LL/NN/OO/PP – Integrated Miniaturized Materials-From Self-Assembly to Device Integration , 2010 , 1272-PP07-04
Copyright
Copyright © Materials Research Society 2010

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