Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2025-01-04T02:54:34.608Z Has data issue: false hasContentIssue false
  • English
  • Français

Physiological Cultured Skin Substitutes for Wound Healing

Published online by Cambridge University Press:  15 February 2011

Craig Halberstadt ,
Phil Anderson ,
Ronnda Bartel ,
Ron Cohen  and
Gail Naughton
Craig Halberstadt
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Phil Anderson
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Ronnda Bartel
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Ron Cohen
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Gail Naughton
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Get access

Abstract

Physiological dermal and dermal-epidermal skin analogs have been developed in our laboratory using a novel technology for three-dimensional tissue culture. Human neonatal dermal fibroblasts are seeded on a biodegradable mesh made of polyglycolic or polyglactic acid (PGAIPGL). As the fibroblasts proliferate, they stretch across the mesh openings and secrete growth factors and human dermal matrix proteins, including collagen types I & HI and elastin. This process forms a metabolically active, three-dimensional dermal tissue around the mesh scaffolding. The mesh fibers are hydrolyzed over time and is completely resorbed in vivo within four to eight weeks. Multiple sheets of the PGA/PGL-dermal analog are grown simultaneously in a closed, continuous media-flow system, also developed in our laboratory. After attaining confluence, the dermal sheets may be seeded with keratinocytes, to create a living dermal-epidermal composite tissue.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 323
Copyright
Copyright © Materials Research Society 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Butler, M. Serum-free Media. p. 91–108. In: Thilly, W.G. (ed.), Mammalian Cell Technology, Butterworths, Boston, MA, 1986.Google Scholar
2. Hayashi, I. and Sato, G.H..Replacement of Serum by Hormones Permits Growth of Cells in a Defined Medium, Nature, 259: 132–134, 1976.Google Scholar
3. Griffiths, J.B. Serum and Growth Factors in Cell Culture Media-An Introductory Review, Develop. biol. Standard., 66: 155–160, 1985.Google Scholar
4. Maurer, H.R. Towards Chemically-defined, Serum-free Media for Mammalian Cell Culture. p. 13–31. In: Freshney, R.I. (ed.), Animal Cell Culture: A Practical Approach, IRL Press, Washington, D.C., 1986 Google Scholar
5. Chandler, J.P. Cultivation of Mammalian Cells in Serum-free Medium, ABL, 18–28, January, 1990.Google Scholar
6. Griffiths, B. Perfusion Systems for Cell Cultivation. p. 217–250. In: Lubiniecki, A.S. (ed.), Large-Scale Mammalian Cell Culture Technology, Marcel Dekker, Inc., New York, N.Y., 1990.Google ScholarPubMed
7. Feder, J. Development of Large-Scale Mammalian Cell Perfusion Culture Systems. p. 454–483. In: Spier, R.E. and Griffiths, J.B. (ed.), Modern Approaches to Animal Cell Technology, Butterworth, London, 1987.Google Scholar
8. Feder, J. The Development of Large-Scale Animal Cell Perfusion Culture Systems. p. 125–152. In: Mizrahi, A. (ed.), Upstream Processes: Equipment and Techniques, Alan R. Liss, Inc., New York, N.Y., 1988.Google ScholarPubMed
9. Oka, M.S. and Rupp, R.G.. Large-Scale Animal Cell Culture: A Biological Perspective. p. 71–92. In: Lubiniecki, A.S. (ed.), Large-Scale Mammalian Cell Culture Technology, Marcel Dekker, Inc., New York, N.Y., 1990.Google Scholar
10. Glacken, M.W., Fleischaker, R.J., and Sinskey, A.J.. Large-scale Production of Mammalian Cells and Their Products: Engineering Principles and Barriers to Scale-up. Annals of the New York Academy of Sciences, 413: 355–372, 1983.Google Scholar
11. Van Brunt, I. Artificial Organs From Culture, Bio/Technology, 9: 136–137, 1991.Google Scholar
12. Naughton, G.K., Jacob, L., and Naughton, B.A., A Physiological Skin Model for in vitro Toxicity Studies. In: Alternative Methods in Toxicology, Vol.7. In Vitro Toxicology: New Directions, Goldberg, A.M., Editor, Mary Ann Liebert Publishers, New York, 1989, pp. 183–189.Google Scholar
13. Naughton, B.A., Sibanda, B., and Naughton, G.K. Long-Term Liver Cell Cultures as Potential Substrates for Toxicity Assessment. In: In Vitro Toxicology: Mechanisms and New Technology, Goldberg, A.M., Editor, Mary Ann Liebert Publishers, New York, 1991, pp. 193–202.Google Scholar
14. Naughton, B.A., Preti, R.A., and Naughton, G.K., Hematopoiesis on Nylon Mesh Templates. I. Long-term Culture of Rat Bone Marrow Cells, J. Med. 18:219–250, 1987.Google Scholar
15. Naughton, B.A., and Naughton, G.K., Modulation of Long-term Bone Marrow Culture by Stromal Support Cells, In: Cellular and Molecular Controls of Hematopoiesis, Orlic, D., ed., Ann. N.Y. Acad. Sci. 554:125–140, 1989.Google Scholar
16. Naughton, B.A., Jacob, L., and Naughton, G.K., A Three-dimensional Culture System for the Growth of Hematopoietic Cells, Progs. Clin., Biol. Res., Vol. 333: Bone marrow processing and purging, Gross, S., Gee, A.P., and Worthington-White, D.A., eds, 435–445, 1990.Google Scholar
17. Horvath, B.E. Mammalian Cell Culture Scale-Up: Is Bigger Better?, Bio/Technology, 7: 468–469, 1989.Google Scholar
18. Tharakan, J.P., Galllagher, S.L., and Chau, P.C.. Hollow-Fiber Bioreactors for Mammalian Cell Culture. p. 153–184. In: Mizrahi, A. (ed.), Upstream Processes: Equipment and Techniques, Alan R. Liss, Inc., New York, N.Y., 1988.Google Scholar
19. Knazek, R.A. Solid Tissue Masses Formed In Vitro from Cells Cultured on Artificial Capillaries, Federation Proceedings, 33(8): 1978–1981, 1974.Google Scholar
20. Knight, P. Hollow Fiber Bioreactors for Mammalian Cell Culture, Bio/Technology, 7: 459–461, 1989.Google Scholar
21. Gallagher, S.L., Tharakan, J.T., and Chau, P.C.. An Intercalated-Spiral Wound Hollow Fiber Bioreactor for the Culture of Mammalian Cells, Biotechnology Techniques, 1(2): 91–96, 1987.Google Scholar
22. Tharakan, J.P. and Chau, P.C.. A Radial Flow Hollow Fiber Bioreactor for the Large-Scale Culture of Mammalian Cells, Biotechnology and Bioengineering, 18: 329–342, 1986.Google Scholar
23. Lim, F. and Moss, R.D.. Microencapsulation of Living Cells and Tissues, Journal of Pharmaceutical Sciences, 70(4): 351–354, 1981.Google ScholarPubMed
24. Nilsson, K., Scheirer, W., Merten, O.W. Ostberg, L., Liehl, E., Katinger, H.W.D. and Mosbach, K.. Entrapment of Animal Cells for Production of Monoclonal Antibodies and Other Biomolecules, Nature, 302: 629–630, 1983.CrossRefGoogle ScholarPubMed
25. Lim, F. Microencapsulation of Living Mammalian Cells, Advances in Biotechnological Processes, 7:185–197, 1988.Google ScholarPubMed
26. Tyler, J.E. Microencapsulation of Mammalian Cells. p. 343–361. In: Lubiniecki, A.S. (ed.), Large-Scale Mammalian Cell Culture Technology, Marcel Dekker, Inc., New York, N.Y., 1990.Google Scholar
27. Runstadler, P.W. Jr.,, Tung, A.S., Hayman, E.G., Ray, N.G., Sample, J.V.G., and DeLucia, D.E.. Continuous Culture with Macroporous Matrix, Fluidized Bed Systems. p. 363–391. In: Lubiniecki, A.S. (ed.), Large-Scale Mammalian Cell Culture Technology, Marcel Dekker, Inc., New York, N.Y., 1990.Google Scholar
28. Seaver, S.S. Culture Method Affects Antibody Secretion of Hybridoma Cells. p. 49–71. In Seaver, S.S. (ed.), Commercial Production of Monoclonal Antibodies: A Guide for Scale-up, Marcel Dekker, Inc., New York, N.Y., 1987.Google Scholar
29. Fleischmajer, R., Cantard, P.; Collagen and Microfibril Formation in Three-Dimensional Mesh Dermal Fibroblast Cultures, J. Invest. Derm., 96(4):540, 1991.Google Scholar
30. Audus, K.L., Bartel, R.L., Hidalgo, I.J., Borchardt, R.T.; The Use of Cultured Epithelial and Endothelial Cells for Drum Transport and Metabolism Studies, Pharm Res, 1990, 7:435–451 CrossRefGoogle Scholar
31. Slivka, S.R., Landeen, L.L., Donelly, T., Zimber, M., Naughton, G.K., Bartel, R.L.; Characterization of a Three-Dimensional Co-culture of Neonatal Human Fibroblasts and Keratinocytes. J. Invest. Derm., 96(4):554, 1991.Google Scholar
32. Borenfreund, D., Puerner, J.; A Simple Quantitative Procedure Using Monolayer Cultures for Cytotoxicity Assays (HTD/NR-90). T. Tissl. Cult. Meth. 9(1):7–9, 1984.Google Scholar
33. Hansbrough, J.F., Cooper, M.L., Cohen, R. Spielvogel, R., Greenleaf, G., Bartel, R., and Naughton, G. Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice, Surgery 110: 1991.Google Scholar
34. Cooper, M.L., Hansbrough, J.F., Spielvogel, R.L., Cohen, R., Bartel, R.L., Naughton, G. In Vivo Optimization of a Living Dermal Substitute Employing Cultured Human Fibroblasts on a Biodegradable Polyglycolic Acid or Polyglactin Mesh, Biomaterials, 12: 243–248, 1991.CrossRefGoogle ScholarPubMed
35. Cohen, R., Zimber, M., Hansbrough, J.F., Fung, Y.C., Debes, J., Skalak, R. Tear Strength Properties of a Novel Cultured Dermal Tissue Model, Presented at Biomedical Engineering Conference, October 11–14, 1991, Charlottesville, VA.Google Scholar