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Physiological Cultured Skin Substitutes for Wound Healing

Published online by Cambridge University Press:  15 February 2011

Craig Halberstadt ,
Phil Anderson ,
Ronnda Bartel ,
Ron Cohen  and
Gail Naughton
Craig Halberstadt
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Phil Anderson
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Ronnda Bartel
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Ron Cohen
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
Gail Naughton
Affiliation:
Advanced Tissue Sciences, La Jolla, CA
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Abstract

Physiological dermal and dermal-epidermal skin analogs have been developed in our laboratory using a novel technology for three-dimensional tissue culture. Human neonatal dermal fibroblasts are seeded on a biodegradable mesh made of polyglycolic or polyglactic acid (PGAIPGL). As the fibroblasts proliferate, they stretch across the mesh openings and secrete growth factors and human dermal matrix proteins, including collagen types I & HI and elastin. This process forms a metabolically active, three-dimensional dermal tissue around the mesh scaffolding. The mesh fibers are hydrolyzed over time and is completely resorbed in vivo within four to eight weeks. Multiple sheets of the PGA/PGL-dermal analog are grown simultaneously in a closed, continuous media-flow system, also developed in our laboratory. After attaining confluence, the dermal sheets may be seeded with keratinocytes, to create a living dermal-epidermal composite tissue.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 323
Copyright
Copyright © Materials Research Society 1992

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