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Porous Polymer-Ceramic Systems for Tissue Engineering Support the Formation of Mineralized Bone Matrix

Published online by Cambridge University Press:  15 February 2011

C. T. Laurencin ,
M. A. Attawia ,
H. M. Elgendy  and
M. Fan
C. T. Laurencin
Affiliation:
The Helen I. Moorehead-Laurencin Biomaterials Research Laboratory, Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA Department of Orthopaedic Surgery, The Medical College of Pennsylvania and Hahnemann University, Philadelphia, PA Department of Chemical Engineering, Drexel University, Philadelphia, PA
M. A. Attawia
Affiliation:
The Helen I. Moorehead-Laurencin Biomaterials Research Laboratory, Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA Department of Orthopaedic Surgery, The Medical College of Pennsylvania and Hahnemann University, Philadelphia, PA
H. M. Elgendy
Affiliation:
The Helen I. Moorehead-Laurencin Biomaterials Research Laboratory, Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA
M. Fan
Affiliation:
The Helen I. Moorehead-Laurencin Biomaterials Research Laboratory, Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA
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Abstract

Osteoblast cell differentiation and bone formation using rat calvaria cells were studied on the surface of a porous 3-dimensional polymer matrix of 50:50 poly (lactide-co-glycolide) (PLAGA)/ hydroxyapatite (HA). Cell proliferation was determined at 24 hr, 3, 7, 14, and 21 days. Exponential growth was observed during the first week and a steady increase in the population continued until 21 days after cells were seeded. Cell growth kinetics on PLAGA/HA showed an exponential pattern. Environmental scanning electron microscopy revealed the formation of multilayers of cells growing throughout the polymer matrices by day 7 and through day 21. Alkaline phosphatase (AP) activity was demonstrated after 21 days of culture on PLAGA/HA using a commercial diagnostic kit. Light microscopy observations of experiment cultures revealed active osteoblastic cells forming a characteristic mineralized matrix in the presence of β-glycerophosphate as a phosphate donor. Mineralization was not seen when the matrix alone was incubated with the reagent, indicating that the mineralization was due to the cells and not the HA in the matrix. These results suggested that the 3-dimensional PLAGA/HA matrix studied provided an excellent matrix for bone cell differentiation and mineralization in vitro and therefore may be a good candidate as a synthetic implant for bone regeneration.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 414: Symposium Z – Thin Films and Surfaces for Bioactivity and Biomedical Applications , 1995 , 157
Copyright
Copyright © Materials Research Society 1996

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