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Production of a Novel, Self-Assembled, Collagenous Matrix Mimicking the Hierarchical Structure of Native Aligned Connective Tissue

Published online by Cambridge University Press:  15 February 2011

G. D. Pins ,
D. L. Christiansen ,
R. Patel  and
F. H. Silver
G. D. Pins
Affiliation:
University of Medicine and Dentistry of New Jersey - Robert Wood Johnson Medical School, Department of Pathology/Division of Biomaterials, 675 Hoes Lane, Piscataway, New Jersey 08854, pins@rwja.umdnj.edu
D. L. Christiansen
Affiliation:
University of Medicine and Dentistry of New Jersey - Robert Wood Johnson Medical School, Department of Pathology/Division of Biomaterials, 675 Hoes Lane, Piscataway, New Jersey 08854, pins@rwja.umdnj.edu
R. Patel
Affiliation:
University of Medicine and Dentistry of New Jersey - Robert Wood Johnson Medical School, Department of Pathology/Division of Biomaterials, 675 Hoes Lane, Piscataway, New Jersey 08854, pins@rwja.umdnj.edu
F. H. Silver
Affiliation:
University of Medicine and Dentistry of New Jersey - Robert Wood Johnson Medical School, Department of Pathology/Division of Biomaterials, 675 Hoes Lane, Piscataway, New Jersey 08854, pins@rwja.umdnj.edu
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Abstract

The primary goal of the biomaterials scientist and tissue engineer is to create a biocompatible implant which mimics the mechanical and morphological properties of the tissue being replaced. In vitro experimentation has documented the propensity of soluble type I collagen to self-assemble and form microscopic collagen fibrils with periodic banding analogous to native collagen fiber. Our laboratory has further investigated in vitro self-assembly by incorporating several of the “natural” processes into a multi-step fiber formation procedure which generates macroscopic collagen fiber from its molecular constituents. Results of uniaxial tensile tests and ultrastructural analyses indicate that these coextruded and stretched collagen fibers have mechanical properties and fibrillar substructure comparable to that observed in native collagen fiber.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 414: Symposium Z – Thin Films and Surfaces for Bioactivity and Biomedical Applications , 1995 , 11
Copyright
Copyright © Materials Research Society 1996

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