Hostname: page-component-6bf8c574d5-b4m5d Total loading time: 0 Render date: 2025-02-22T08:22:52.486Z Has data issue: false hasContentIssue false
  • English
  • Français

Review Molecular Mechanism of Bacterial Magnetite Formation and Its Application

Published online by Cambridge University Press:  01 February 2011

Tadashi Matsunaga  and
Yoshiko Okamura
Tadashi Matsunaga
Affiliation:
Department of Biotechnology, Tokyo University of Agriculture and Technology Naka-Cho, Koganei, Tokyo 184-8588, Japan
Yoshiko Okamura
Affiliation:
Department of Biotechnology, Tokyo University of Agriculture and Technology Naka-Cho, Koganei, Tokyo 184-8588, Japan
Get access

Abstract

Nano-technology has been identified as an area which will bring about new evolutions in materials, devices and processes. The challenges of nano-biotechnology entail manufacturing more sophisticated and highly efficient biosensors and biomaterials at the nano-scale level for use in interdisciplinary fields. Here, we introduce a biomaterial produced by magnetic bacteria, bacterial magnetic particles (BMPs), and the molecular architecture technique we have used for its application.

Magnetic bacteria synthesize intracellular magnets which are encapsulated by lipid bilayer membranes. Sizes of BMPs vary from 50 - 100 nm in diameter, and number over 10 per cell. BMPs are composed of magnetite (Fe3O4) with a single magnetic domain. Easy aqueous dispersion of BMPs enable development of highly sensitive chemiluminescence enzyme immunoassays by the chemical coupling of antibodies on BMP surfaces. BMPs can likewise be used as drug delivery systems employing magnetoliposomes with high capture volumes. We previously reported a technique for preparing recombinant BMPs on which proteins were displayed by gene-fusion. We furthermore applied such recombinant BMPs to biotechnologically-important issues, including novel bioassay platforms for medicine and environmental management. We envisage the production of more refined chemicals and agents through expression on BMPs by gene-fusion followed by simple purification using magnet.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 724: Symposium N – Biological and Biomimetic Materials Properties to Function , 2002 , N1.4
Copyright
Copyright © Materials Research Society 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Blakemore, R. P., Science 190, 377 (1975).Google Scholar
2. Matsunaga, T., Tadokoro, F. and Nakamura, N., IEEE Trans. Magn, 26, 1557 (1991).Google Scholar
3. Matsunaga, T., Sakaguchi, T. and Tadokoro, F., Appl. Microbiol. Biotechnol. 35, 651 (1991).Google Scholar
4. Sakaguchi, T., Burgess, J. G. and Matsunaga, T., Nature 365, 47 (1993).Google Scholar
5. Balkwill, D. L., Maratea, D. and Blakemore, R. P., J. Bacteriol. 141, 1399 (1980).Google Scholar
6. Nakamura, N., Hashimoto, K. and Matsunaga, T., Anal. Chem. 63, 268 (1991).Google Scholar
7. Nakamura, N., Burgess, J. G., Yagiuda, K., kudo, K., Sakaguchi, S. and Matsunaga, T., Anal. Chem. 65, 2036 (1993).Google Scholar
8. Nakamura, N. and Matsunaga, T., Anal. Chim. Acta, 281, 585 (1993).Google Scholar
9. Sode, K., Kudo, S., Sakaguchi, T., Nakamura, N. and Matsunaga, T., Biotechnol. Tech. 7, 688 (1993).Google Scholar
10. Takeyama, H., Yamazawa, A., Nakamura, C. and Matsunaga, T., Biotechnol. Tech. 9, 355 (1995).Google Scholar
11. Matsunaga, T., Nakamura, C., Burgess, J. G. and Sode, K., J. Bacteriol. 174, 2748 (1992).Google Scholar
12. Blakemore, R. P. and Blakemore, N. A., in Iron biominerals, ed. Frankel, R. B. and Blakemore, R. P. (Plenum Press, 1991) pp. 51–67.Google Scholar
13. Blakemore, R. P., Ann. Rev. Microbiol. 36, 217 (1982).Google Scholar
14. Blakemore, R. P., Blakemore, N. A., Bazylinski, D. A. and Moench, T. T., in Bergey's manual of systematic bacteriology, vol. 3, edited by Staley, J. T., Bryant, M. P., Pfenning, N. and Holt, J. G., (Wiliams & Wilkins, Baltimore, 1989) pp. 1882–1889.Google Scholar
15. Esquivel, D. M. S. and Barros, H. G. P. Linns de, J. Exp. Biol. 121, 153 (1986).Google Scholar
16. Mann, S., Sparks, N. H. C. and Borad, R. G., Adv. Microb. Physiol. 31, 125 (1990).Google Scholar
17. Oberhack, M., Sussmuth, R. and Frank, H., Z. Naturforsch. 42c, 300 (1987).Google Scholar
18. Sparks, N. H. C., Courtaux, L., Mann, S., and Borad, R. G., FEMS Microbiol. Lett. 37, 305 (1986).Google Scholar
19. Matsunaga, T. and Kamiya, S., Appl. Microbiol. Biotechnol. 26, 328 (1987).Google Scholar
20. Thornhill, R. H., Burgess, J. G., Sakaguchi, T. and Matsunaga, T, FEMS Microbiol. Lett. 115, 169 (1993).Google Scholar
21. Fassbinder, J. W. E. Stanjek, H. and Vail, H., Nature 343, 161 (1990).Google Scholar
22. Stolz, J. F., Chang, S. -B. R. and Kirschvink, J. L., Nature 321, 849 (1986).Google Scholar
23. Schuler, D. and Baeuerlein, E., in Transition metals in microbial metabolism, ed. Winkelmann, G. and Carrano, C. J. (Harwood Academic Publishers, Amsterdam, The Netherlands, 1997)Google Scholar
24. Stolz, J. F., J. Gen Microbiol. 139, 1663 (1993).Google Scholar
25. Bazylinski, D. A., ASM News 61, 337 (1995).Google Scholar
26. Schuler, D. and Frankel, R. B., Appl. Microbiol. Biotechnol. 52, 464 (1999).Google Scholar
27. Matsunaga, T., Sakaguchi, T., Sode, K., Kudo, S. and Nakamura, N., in Proceedings of the3rd IUMRS international conference on advanced materials, edited by Aoki, H. et al. (Advanced materials '93 II/A, Kawasaki, Japan, 1993) pp. 449–454.Google Scholar
28. Gorby, Y. A., Beveridge, T. J. and Blakemore, R. P., J. Bacteriol. 170, 834 (1988).Google Scholar
29. Matsunaga, T., Tsujimura, N., Okamura, Y. and Takeyama, H., Biochem. Biophys. Res. Commun. 268, 932 (2000).Google Scholar
30. Okamura, Y., Takeyama, H. and Matsunaga, T., Appl. Biochem. Biotech. 84-86, 441 (2000).Google Scholar
31. Nakamura, C., Burgess, J. G., Sode, K. and Matsunaga, T., J. Biol. Chem. 270, 28392 (1995).Google Scholar
32. Nakamura, C., Kikuchi, T., Burgess, J. G. and Matsunaga, T., J. Biochem. 118, 23 (1995).Google Scholar
33. Okamura, Y., Takeyama, H. and Matsunaga, T., J. Biol. Chem. 276, 48183 (2001).Google Scholar
34. Matsunaga, T., Sato, R., Kamiya, S., Tanaka, T. and Takeyama, H., J. Magn. Magn. Mater. 194, 126 (1999).Google Scholar
35. Tanaka, T. and Matsunaga, T., Anal. Chem. 72, 3518 (2000).Google Scholar
36. Yang, C. -D., Takeyama, H., Tanaka, T. and Matsunaga, T., Enz. Microb. Technol. 29, 13 (2001).Google Scholar
37. Tanaka, T. and Matsunaga, T., Biosens. & Bioelectronics, 16, 1089 (2001).Google Scholar