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Structural Dynamics of a Single Photoreceptor Protein Molecule Monitored With Surface-Enhanced Raman Scattering Substrates

Published online by Cambridge University Press:  01 February 2011

Kushagra Singhal ,
Karthik Bhatt ,
Zhouyang Kang ,
Wouter Hoff ,
Aihua Xie  and
A. Kaan Kalkan
Kushagra Singhal
Affiliation:
singhal@okstate.edu, Oklahoma State University, Stillwater, Functional Nanomaterials Lab, Department of Mechanical and Aerospace Engineering, 218 Engineering North, Oklahoma State University, Stillwater, OK, 74078, United States, 405 744 3719
Karthik Bhatt
Affiliation:
karthik.bhatt@okstate.edu, Oklahoma State University, Functional Nanomaterials Lab, Department of Mechanical and Aerospace Engineering, 218 Engineering North, Stillwater, OK, 74078, United States
Zhouyang Kang
Affiliation:
zhouyang.kang@okstate.edu, Oklahoma State University, Department of Physics, 145 Physical Sciences, Stillwater, OK, 74078, United States
Wouter Hoff
Affiliation:
wouter.hoff@okstate.edu, Oklahoma State University, Department of Microbiology and Molecular Genetics, 307 Life Sciences East, Stillwater, OK, 74078, United States
Aihua Xie
Affiliation:
aihua.xie@okstate.edu, Oklahoma State University, Department of Physics, 145 Physical Sciences, Stillwater, OK, 74078, United States
A. Kaan Kalkan
Affiliation:
kaan.kalkan@okstate.edu, Oklahoma State University, Functional Nanomaterials Lab, Department of Mechanical and Aerospace Engineering, 218 Engineering North, Stillwater, OK, 74078, United States
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Abstract

Photoactive yellow protein (PYP) is a small cytosolic photoreceptor that actuates the negative phototactic response in its host organism Halorhodospira halophila. It has an optical absorption maximum at 446 nm (blue light). We report an initial study of the photocycle of PYP at the single molecule level using “high enhancement factor” surface-enhanced Raman scattering (SERS)-active nanostructures with 514 nm laser excitation. The SERS-active “nanometal-on-semiconductor” structures are prepared employing a redox technique on thin germanium films, coated on glass slides. Single molecule spectra are observed in terms of sudden appearance of discernable Raman peaks with spectral fluctuations. The single molecule spectra capture protonation, photo-isomerization, and H-bond breaking - the steps that are instrumental in the photocycle of PYP. This is indicative of single PYP molecules diffusing to high-enhancement-factor SERS sites, and undergoing photo-cycle under 514 nm excitation.

Keywords

nanostructureAgRaman spectroscopy
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1077: Symposium L – Functional Plasmonics and Nanophotonics , 2008 , 1077-L10-04
Copyright
Copyright © Materials Research Society 2008

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