Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-29T11:49:30.107Z Has data issue: false hasContentIssue false
  • English
  • Français

Terminal phosphate group influence on DNA - TiO2 nanoparticle interactions

Published online by Cambridge University Press:  31 January 2011

Zachary Rice ,
Nathaniel C Cady  and
Magnus Bergkvist
Zachary Rice
Affiliation:
PRice@uamail.albany.edurice.zach@gmail.com
Nathaniel C Cady
Affiliation:
ncady@uamail.albany.edu, University at Albany, CNSE, 257 Fuller Road, Albany, New York, 12203, United States, 518 437 8686
Magnus Bergkvist
Affiliation:
mBergkvist@uamail.albany.edu, United States
Get access

Abstract

Immobilization of DNA/RNA, onto various metal and metal oxide surfaces is of great importance for the development of future microarray, gene mapping, DNA sequencing, nanoparticle targeting, and sensor applications. Attachment of DNA to solid interfaces typically occurs through either electrostatic interactions or covalent bonds to functional groups introduced to nucleic acid termini. Previously, we and others have demonstrated that alkanephosphates and terminal phosphate groups present on nucleic acids play an important role in the interaction with group IV metal oxides such as zirconium and hafnium, providing a stable linkage to the surface. Titanium dioxide (TiO2), which is frequently employed in various nanoscale applications, belongs to the same group and similar interactions with phosphate are expected. Various adsorption studies have demonstrated binding of nucleic acids to TiO2 surfaces, although the influence of terminal phosphate versus electrostatic interaction (via the DNA/RNA backbone) on the surface interaction is unclear. The research presented here investigates the effect of nucleic acid length, presence of terminal phosphates, and differences between dsDNA and ssDNA on their binding to TiO2 nanoparticles. TiO2 nanoparticles (20 nm) were used to study the adsorption of Lambda DNA (˜48 kbp), and shorter (21 bp) ssDNA and dsDNA oligonucleotides with and without a 5’ phosphate group. Initial adsorption of DNA to nanoparticles was calculated via UV absorption. Results showed that all types of nucleic acids (Lamda DNA, ssDNA and dsDNA) initially bind to nanoparticles, independent of molecular weight single/double strandedness, or phosphorylation state. The total amount of DNA initially adsorbed to nanoparticles (ng/particle) differs between ssDNA and dsDNA, as well as the length of the DNA used. These results show that nucleic acid interactions with TiO2 nanoparticles are not dependent upon the presence of a terminal phosphate group. These results provide valuable data for future applications based on DNA-nanoparticle constructs including nanoelectronics, photovoltaics, and biotemplated synthesis of semiconducting materials.

Keywords

adsorptionTinanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1236: Symposium SS – Biosurfaces and Biointerfaces , 2009 , 1236-SS05-15
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Chang, H. and Tzeng, W. W.-C., A Combined Conjugation and Hybridization Technology for Different Types of DNA and Nanoparticles. Materials Transactions, 2008. 49(6): p. 1467–1473.Google Scholar
2 Zhu, Z., Zhu, T., and Liu, Z., Raman scattering enhancement contributed from individual gold nanoparticles and interparticle coupling. Nanotechnology, 2004. 15(3): p. 357–364.Google Scholar
3 Lobert, P.E. et al. , Immobilization of DNA on CMOS compatible materials. mmobilization Sensors and Actuators B: Chemical, 2003. 92(1-2): p. 90–97.Google Scholar
4 Pawsey, S. et al. , 1H Fast MAS NMR Studies of Hydrogen-Bonding Interactions in Self-Assembled Monolayers. Journal of the American Chemical So Society, 2003. ciety, 125(14): p. 4174–4184.Google Scholar
5 Nonglaton, G. et al. , New Approach to Oligonucleotide Microarrays Using Zirconium Phosphonate-Modified Surfaces. Journal of the American Chemical Society, 2004. 126(5): p. 1497–1502.Google Scholar
6 Xu, X. X.-H. et al. , Immobilizat Immobilization of DNA on an Aluminum(III) Alkanebisphosphonate ion Thin Film with Electrogenerated Chemiluminescent Detection. Journal of the American Chemical Society, 2002. 116 (18): p. 8386–8387.Google Scholar
7 Fahrenkopf, N. et al. , Phosphate Phosphate-dependent DNA Immobilization on Hafn Hafnium Oxide for ium Bio Bio-Sensing Applications. Proceedings of the Materials Research Society, 2009.Google Scholar
8 Rusop, M. et al. , Optical Band Gap Excitation and Photoelectron Generation in Titanium Dioxide Dioxide-Based Solid State Solar Cells. Surface Review & Letters, 2005. 12(5/6): p. 681–689.Google Scholar
9 Kim, Y.J., Chai, S.Y., and Lee, W.I., Control of TiO2 Structures from Robust Hollow Microspheres to Highly Dispersible Nanoparticles in a Tetrabutylammonium Hydroxide Solution. Langmuir, 2007. 23(19): p. 9567–9571.Google Scholar