Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-10T08:50:55.642Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of hydrogel microstructures as templates for protein immobilization

Published online by Cambridge University Press:  01 February 2011

Won-Gun Koh  and
Dae Nyun Kim
Won-Gun Koh
Affiliation:
wongun@yonsei.ac.kr, Yonsei University, Chemical Engineering, 134 Sinchondong, Seodaemoongu,, Seoul, 120-749, Korea, Republic of
Dae Nyun Kim
Affiliation:
deyuni@empal.com, Yonsei University, Chemical Engineering, Seoul, 120-749, Korea, Republic of
Get access

Abstract

In this study, protein micropatterns were created on the surface of three-dimensional hydrogel microstructures. Poly(ethylene glycol)(PEG)-based hydrogel microstructures were fabricated on a glass substrate using a poly(dimethylsiloxane) (PDMS) replica as a molding insert and photolithography. The lateral dimension and height of the hydrogel microstructures were easily controlled by the feature size of the photomask and depth of the PDMS replica, respectively. Bovine serum albumin (BSA), a model protein, was covalently immobilized to the surface of the hydrogel microstructure via a 5-azidonitrobenzoyloxy N-hydroxysuccinimide bifunctional linker, which has a phenyl azide group and a protein-binding N-hydroxysuccinimide group on either end. The immobilization of BSA on the PEG hydrogel surface was demonstrated with XPS by confirming the formation of a new nitrogen peak, and the selective immobilization of fluorescent-labeled BSA on the outer region of the three-dimensional hydrogel micropattern was demonstrated by fluorescence. A hydrogel microstructure could immobilize two different enzymes separately, and sequential bienzymatic reaction was demonstrated by reacting glucose and Amplex Red with a hydrogel microstructure where glucose oxidase was immobilized on the surface and peroxidase was encapsulated.

Keywords

sensorbiomedicalmicrostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1095: Symposium EE – Responsive Biomaterials for Biomedical Applications , 2008 , 1095-EE08-08
Copyright
Copyright © Materials Research Society 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Blawas, A.S., Reichert, W.M., Biomaterials 19 (1998) 595.Google Scholar
2. Chovan, T., Guttman, A., Trends Biotechnol. 20 (2002) 116.Google Scholar
3. Mitchell, P., Nat. Biotechnol. 20 (2002) 225.Google Scholar
4. Jakeway, S.C., Mello, A.J. de, Russell, E.L., Fresenius, J. Anal. Chem. 366 (2000) 525.Google Scholar
5. Angenendt, P., Drug Discov. Today 10 (2005) 503.Google Scholar
6. Balakirev, M.Y., Porte, S., Vernaz-Gris, M., Berger, M., Arie, J.P., Fouque, B., Chatelain, F., Anal. Chem. 77 (2005) 5474.Google Scholar
7. Ginger, D.S., Zhang, H., Mirkin, C.A., Angew. Chem. Int. Ed. 43 (2004) 30.Google Scholar
8. Glokler, J., Angenendt, P., J. Chromatogr. B 797 (2003) 229.Google Scholar
9. Kane, R.S., Takayama, S., Ostuni, E., Ingber, D.E., Whitesides, G.M., Biomaterials 20 (1999) 2363.Google Scholar
10. Kiyonaka, S., Sada, K., Yoshimura, I., Shinkai, S., Kato, N., Hamachi, I., Nat. Mater. 3 (2004) 58.Google Scholar
11. Zhang, S., Nat. Mater. 3 (2004) 7.Google Scholar
12. Koh, W.G., Revzin, A., Pishko, M.V., Langmuir 18 (2002) 2459.Google Scholar
13. Liu, V.A., Bhatia, S.N., Biomed. Microdevices 4 (2002) 257.Google Scholar
14. Revzin, A., Russell, R.J., Yadavalli, V.K., Koh, W., Deister, C., Hile, D.D., Mellott, M.B., Pishko, M.V., Langmuir 17 (2001) 5440.Google Scholar
15. Russell, R.J., Pishko, M.V., Simonian, A.L., Wild, J.R., Anal. Chem. 71 (1999) 4909.Google Scholar
16. Hahn, M.S., Taite, L.J., Moon, J.J., Rowland, M.C., Ruffino, K.A., West, J.L., Biomaterials 27 (2006) 2519.Google Scholar
17. Heo, J., Crooks, R.M., Anal. Chem. 77 (2005) 6843.Google Scholar