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Agrobacterium tumefaciens-mediated transformation of Fusarium circinatum

Published online by Cambridge University Press:  21 May 2001

Sarah F. COVERT ,
Pratibha KAPOOR ,
Mei-ho LEE ,
Angela BRILEY  and
C. Joseph NAIRN
Sarah F. COVERT
Affiliation:
Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA. E-mail: covert@arches.uga.edu
Pratibha KAPOOR
Affiliation:
Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA. E-mail: covert@arches.uga.edu Present address: Department of Comparative Medicine, University of Alabama at Birmingham, Birmingham, AL 35294-0019, USA.
Mei-ho LEE
Affiliation:
Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA. E-mail: covert@arches.uga.edu
Angela BRILEY
Affiliation:
Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA. E-mail: covert@arches.uga.edu
C. Joseph NAIRN
Affiliation:
Department of Botany, University of Georgia, Athens, GA 30602, USA.
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Abstract

The development of a transformation system is a significant technical hurdle in the study of many filamentous fungi. Recently Agrobacterium tumefaciens was shown to transform several filamentous fungi, but until now only one independent confirmation of this promising finding has been published. Fusarium circinatum (teleomorph Gibberella circinata) is an important pathogen of pine that has not been transformed previously. A. tumefaciens strain AGL1(pPK2) transformed three different isolates of F. circinatum with an efficiency of 2–150 transformants 10−5 conidia. The T-DNA was integrated into the genome and was stable through mitotic and meiotic cell divisions. Most F. circinatum transformants contained a single T-DNA copy. Half of the tested transformants also contained non-T-DNA vector sequences. These findings should facilitate future analysis of F. circinatum pathogenicity and stimulate wider use of this valuable transformation method in fungal research.

Information

Type
Research Article
Information
Mycological Research , Volume 105 , Issue 3 , March 2001 , pp. 259 - 264
Copyright
© The British Mycological Society 2001

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