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In vivo observations of the arbuscular mycorrhizal fungus Glomus mosseae in roots by confocal laser scanning microscopy

Published online by Cambridge University Press:  01 March 1999

HORST VIERHEILIG ,
ANNETTE BÖCKENHOFF ,
MICHAEL KNOBLAUCH ,
CHRISTINE JUGE ,
AART J. E. VAN BEL ,
FLORIAN GRUNDLER ,
YVES PICHÉ  and
URS WYSS
HORST VIERHEILIG
Affiliation:
Institut für Phytopathologie, Christian-Albrecht-Universität Kiel, Hermann Rodewald-Strasse 9, 24118 Kiel, Germany Université Laval, Faculté de Foresterie et de Géomatique, CRBF, Pavillon C.-E.-Marchand, Québec G1K 7P4, Canada
ANNETTE BÖCKENHOFF
Affiliation:
Institut für Allgemeine Botanik und Pflanzenphysiologie, Justus-Liebig-Universität, Senckenbergstrasse 17, 35390 Giessen, Germany
MICHAEL KNOBLAUCH
Affiliation:
Institut für Allgemeine Botanik und Pflanzenphysiologie, Justus-Liebig-Universität, Senckenbergstrasse 17, 35390 Giessen, Germany
CHRISTINE JUGE
Affiliation:
Université Laval, Faculté de Foresterie et de Géomatique, CRBF, Pavillon C.-E.-Marchand, Québec G1K 7P4, Canada
AART J. E. VAN BEL
Affiliation:
Institut für Allgemeine Botanik und Pflanzenphysiologie, Justus-Liebig-Universität, Senckenbergstrasse 17, 35390 Giessen, Germany
FLORIAN GRUNDLER
Affiliation:
Institut für Phytopathologie, Christian-Albrecht-Universität Kiel, Hermann Rodewald-Strasse 9, 24118 Kiel, Germany
YVES PICHÉ
Affiliation:
Université Laval, Faculté de Foresterie et de Géomatique, CRBF, Pavillon C.-E.-Marchand, Québec G1K 7P4, Canada
URS WYSS
Affiliation:
Institut für Phytopathologie, Christian-Albrecht-Universität Kiel, Hermann Rodewald-Strasse 9, 24118 Kiel, Germany
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Abstract

Confocal laser scanning microscopy was used for in vivo observations of autofluorescent fungal structures of Glomus mosseae in intact, living rye-grass roots. Clear images of hyphae and collapsed arbuscules could be obtained without staining or sectioning roots. As rye-grass roots are highly transparent, with only a few cell layers above the vascular cylinder, they provide an excellent in vivo system for microscopical confocal laser scanning studies of arbuscular mycorrhizal structures.

Information

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 3 , March 1999 , pp. 311 - 314
Copyright
© The British Mycological Society 1999

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