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Protein expressions during fruit body induction of Flammulina velutipes under reduced temperature

Published online by Cambridge University Press:  09 May 2002

Yuichi SAKAMOTO ,
Akira ANDO ,
Yutaka TAMAI ,
Kiyoshi MIURA  and
Takahashi YAJIMA
Yuichi SAKAMOTO
Affiliation:
Ibaraki Prefectural Forestry Research Institute, Nakamachi To 4692, Nakagun, Ibaraki 311-0122, Japan.
Akira ANDO
Affiliation:
Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Yutaka TAMAI
Affiliation:
Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Kiyoshi MIURA
Affiliation:
Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Takahashi YAJIMA
Affiliation:
Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
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Abstract

The influences of temperature on the fruit body induction of Flammulina velutipes were investigated. The fruit body could be induced after reducing of the ambient temperature under complete darkness, but could not be induced after light irradiation without temperature reduction. Fruit bodies formed under complete darkness elongated without pileus formation (pinhead fruit body). Protein expression patterns were investigated by two-dimensional electrophoresis, and 22 protein spots were found to be newly expressed in the mycelia and fruit bodies after reducing of the ambient temperature, but few protein spots were newly expressed in the mycelia after light irradiation without temperature reduction. Four of the spots had identical N-terminal sequences to polypeptides, Pf1, Pf3, Pfd3, and Pf6 which are previously sequenced as polypeptides expressed specifically during fruit body formation of F. velutipes.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 2 , February 2002 , pp. 222 - 227
Copyright
© The British Mycological Society 2002

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