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Development of a glial network in the olfactory nerve: role of calcium and neuronal activity

Published online by Cambridge University Press:  21 September 2011

Mounir A. Koussa ,
Leslie P. Tolbert  and
Lynne A. Oland
Mounir A. Koussa
Affiliation:
Department of Neuroscience, University of Arizona, Tucson, AZ, USA
Leslie P. Tolbert
Affiliation:
Department of Neuroscience, University of Arizona, Tucson, AZ, USA
Lynne A. Oland*
Affiliation:
Department of Neuroscience, University of Arizona, Tucson, AZ, USA
*
Correspondence should be addressed to: Lynne A. Oland, Department of Neuroscience, University of Arizona, PO Box 210077, Tucson, AZ 85721, USA email: oland@email.arizona.edu
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Abstract

In adult olfactory nerves of mammals and moths, a network of glial cells ensheathes small bundles of olfactory receptor axons. In the developing antennal nerve (AN) of the moth Manduca sexta, the axons of olfactory receptor neurons (ORNs) migrate from the olfactory sensory epithelium toward the antennal lobe. Here we explore developmental interactions between ORN axons and AN glial cells. During early stages in AN glial-cell migration, glial cells are highly dye coupled, dividing glia are readily found in the nerve and AN glial cells label strongly for glutamine synthetase. By the end of this period, dye-coupling is rare, glial proliferation has ceased, glutamine synthetase labeling is absent, and glial processes have begun to extend to enwrap bundles of axons, a process that continues throughout the remainder of metamorphic development. Whole-cell and perforated-patch recordings in vivo from AN glia at different stages of network formation revealed two potassium currents and an R-like calcium current. Chronic in vivo exposure to the R-type channel blocker SNX-482 halted or greatly reduced AN glial migration. Chronically blocking spontaneous Na-dependent activity by injection of tetrodotoxin reduced the glial calcium current implicating an activity-dependent interaction between ORNs and glial cells in the development of glial calcium currents.

Keywords

Calcium currentsManduca sextaantennal nerve
Type
Research Article
Information
Neuron Glia Biology , Volume 6 , Issue 4 , November 2010 , pp. 245 - 261
Copyright
Copyright © Cambridge University Press 2011

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