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Profiles of photosynthetic oxygen-evolution within leaves of Spinacia oleracea

Published online by Cambridge University Press:  01 July 1999

T. HAN ,
T. VOGELMANN  and
J. NISHIO
T. HAN
Affiliation:
Botany Department, University of Wyoming, Laramie, WY 82071, USA
T. VOGELMANN
Affiliation:
Botany Department, University of Wyoming, Laramie, WY 82071, USA
J. NISHIO
Affiliation:
Botany Department, University of Wyoming, Laramie, WY 82071, USA
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Abstract

Oxygen evolution was measured from mesophyll tissues in spinach leaves using a photoacoustic technique. The photosynthetic capacity of individual cell layers was measured by directing microscopic beams of light, 40 μm wide, to cells exposed within a leaf cross section. The resulting profile for oxygen-evolution potential was relatively flat, indicating a uniform capacity for photosynthesis in leaf mesophyll tissues. Two experimental approaches were used to estimate the photosynthetic performance of individual mesophyll cell layers when white light was applied to the adaxial leaf surface. These experiments indicated that oxygen was produced relatively uniformly across the mesophyll and that oxygen evolution increased with irradiance of the white light applied to the leaf surface. The measured profiles for oxygen evolution and capacity are flatter than previous measurements of profiles of fixed carbon and estimates of profiles for absorbed light within spinach leaves.

Keywords

leaf anatomypalisade mesophylloxygen evolutionphotoacousticsphotosynthesisspinach (Spinacia oleracea)structure–function
Type
Research Article
Information
The New Phytologist , Volume 143 , Issue 1 , July 1999 , pp. 83 - 92
Copyright
Trustees of the New Phytologist 1999

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