Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T12:00:58.883Z Has data issue: false hasContentIssue false
  • English
  • Français

Fructo-oligosaccharide content and fructosyltransferase activity during growth of onion bulbs

Published online by Cambridge University Press:  01 May 1997

NORIO SHIOMI ,
SHUICHI ONODERA  and
HIDEKI SAKAI
NORIO SHIOMI
Affiliation:
Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University, 582 Bunkyodai, Midorimachi, Ebetsu, Hokkaido 069, Japan
SHUICHI ONODERA
Affiliation:
Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University, 582 Bunkyodai, Midorimachi, Ebetsu, Hokkaido 069, Japan
HIDEKI SAKAI
Affiliation:
Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University, 582 Bunkyodai, Midorimachi, Ebetsu, Hokkaido 069, Japan
Get access

Abstract

The accumulation of fructo-oligosaccharides and the activities of fructosyltransferase (sucrose[ratio ]sucrose 1F-fructosyltransferase (SST), 1F-fructosyltransferase (1F-FT) and 6G-fructosyltransferase (6G-FT)) in the bulbs of three onion cultivars were investigated from June to September 1993. The total fructo-oligosaccharide content increased from June until August, then decreased in September, except in one cultivar. The levels of neokestose and its related tetrasaccharides (1F, 6G-di-β-D-fructofuranosyl sucrose and 6G (1-β-D-fructofuranosyl)2sucrose) were higher than those of 1-kestose and nystose throughout growth. The activities of 6G-FT, 1F -FT and SST were high in June and July, then decreased; SST activity was very low in September. The activity ratios of 6G-FT to 1F-FT varied between 1·86 and 2·65 over the growth period. Two trisaccharides, three tetrasaccharides and four pentasaccharides were identified, together with a mixture of hexa- and heptasaccharides, all of which were synthesized in vitro from 0·1 M sucrose by an enzyme preparation of onion bulbs harvested in August. Octa- and nonasaccharides other than the saccharides formed from sucrose were also synthesized from 0·1 M 1-kestose or 0·1 M neokestose. All the saccharides produced from sucrose, 1-kestose or neokestose by the crude enzyme prepared from onion bulbs were identical to the saccharides occurring naturally in onion bulbs.

Keywords

Allium cepafructo-oligosaccharidesfructosyltransferase
Type
Research Article
Information
The New Phytologist , Volume 136 , Issue 1 , May 1997 , pp. 105 - 113
Copyright
Trustees of the New Phytologist 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)