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Looking for molecular and physiological markers of osmotic adjustment in sunflower

Published online by Cambridge University Press:  01 September 1997

I. JAMAUX ,
A. STEINMETZ  and
E. BELHASSEN
I. JAMAUX
Affiliation:
SGAP, INRA, 2 place Viala, 34060 Montpellier cedex, France
A. STEINMETZ
Affiliation:
IBMP, CNRS, 12 rue du général Zimmer, 67084 Strasbourg cedex, France
E. BELHASSEN
Affiliation:
SGAP, INRA, 2 place Viala, 34060 Montpellier cedex, France
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Abstract

Two families of Helianthus lines, T− (low desiccation rate) and T+ (low desiccation rate), with contrasting osmotic adjustment (OA), obtained from a divergent selection programme, were compared for several morphological traits, ecophysiological parameters and molecular markers. The physiological analysis in a glasshouse experiment showed that the two families were identical in irrigated conditions except for a single trait, relative water loss (RWL: water loss rate of excised leaves). RWL could be used for these genotypes as a physiological marker of OA. A molecular analysis of the two contrasting lines, using differential screening of cDNA libraries, led to the isolation of three specific cDNAs, one of which, DRS26, showed high sequence homology with a mammalian amino-acid transporter. Subsequent RFLP and STS studies, using DRS26 and RAPD bulked analysis, enabled the identification of putative molecular markers of RWL and OA in T−[ratio ]T+ sunflower.

Keywords

Drought tolerancerelative water lossosmotic adjustmentmolecular markerscandidate genes
Type
Research Article
Information
The New Phytologist , Volume 137 , Issue 1 , September 1997 , pp. 117 - 127
Copyright
© Trustees of the New Phytologist 1997

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