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Altered behaviour of carbohydrate-bound molecules and lipids in areas of the tegument of adult Schistosoma mansoni worms damaged by praziquantel

Published online by Cambridge University Press:  06 April 2009

S. F. Lima ,
L. Q. Vieira ,
A. Hardert  and
J. R. Kusel
S. F. Lima
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
L. Q. Vieira
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
A. Hardert
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
J. R. Kusel
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
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Summary

By using fluorescent probes the distribution and fluid properties of lipid and saccharide-bound molecules was assessed in the tegument of praziquantel (−) treated Schistosoma mansoni adult male worms. Our results show that higher amounts of glycoproteins and/or glycolipids are exposed in damaged areas of the membrane compared with undamaged ones. At damaged regions these molecules present high lateral diffusion coefficient and mobile fraction values which suggests that after praziquantel(−) treatment they are being shed by the worm into the medium. The lateral diffusion coefficient of the lipid analogue 5'-octadecanoyl aminofluorescein is unchanged in damaged or undamaged areas but the mobile fraction is significantly reduced at damaged areas. The immunological significance of these altered surface properties is discussed.

Keywords

Schistosoma mansonimembranefluiditypraziquantelFRAP.
Type
Research Article
Information
Parasitology , Volume 109 , Issue 4 , November 1994 , pp. 469 - 477
Copyright
Copyright © Cambridge University Press 1994

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